Potential of Resveratrol Analogues as Antagonists of Osteoclasts and Promoters of Osteoblasts

• Published in: Calcified tissue international Vol. 87; no. 5; pp. 437 - 449
• Main Authors: Kupisiewicz, Katarzyna, Boissy, Patrice, Abdallah, Basem M., Hansen, Frederik Dagnaes, Erben, Reinhold G., Savouret, Jean-Francois, Søe, Kent, Andersen, Thomas L., Plesner, Torben, Delaisse, Jean-Marie
• Format: Journal Article
• Language: English
• Published: New York Springer-Verlag 01-11-2010; Springer Nature B.V
• Subjects: Animals; Anticarcinogenic Agents - agonists; Anticarcinogenic Agents - therapeutic use; Biochemistry; Biomedical and Life Sciences; Bone Density Conservation Agents - agonists; Bone Density Conservation Agents - therapeutic use; Bones; Cell Biology; Cell Line, Tumor; Cellular biology; Endocrinology; Female; Growth Inhibitors - agonists; Growth Inhibitors - therapeutic use; Humans; Life Sciences; Multiple myeloma; Orthopedics; Osteoblasts - cytology; Osteoblasts - drug effects; Osteoclasts - cytology; Osteoclasts - drug effects; Osteogenesis - drug effects; Osteogenesis - physiology; Osteoporosis; Osteoporosis - drug therapy; Osteoporosis - metabolism; Osteoporosis - physiopathology; Phytochemicals; Rats; Resveratrol; Stilbenes - agonists; Stilbenes - therapeutic use; Treatment Outcome; Osteoblast; Osteoclast; Resveratrol; Multiple myeloma; Resveratrol analogue
• ISSN: 0171-967X; 1432-0827
• DOI: 10.1007/s00223-010-9399-3

The plant phytoalexin resveratrol was previously demonstrated to inhibit the differentiation and bone resorbing activity of osteoclasts, to promote the formation of osteoblasts from mesenchymal precursors in cultures, and inhibit myeloma cell proliferation, when used at high concentrations. In the current study, we screened five structurally modified resveratrol analogues for their ability to modify the differentiation of osteoclasts and osteoblasts and proliferation of myeloma cells. Compared to resveratrol, analogues showed an up to 5,000-fold increased potency to inhibit osteoclast differentiation. To a lesser extent, resveratrol analogues also promoted osteoblast maturation. However, they did not antagonize the proliferation of myeloma cells. The potency of the best-performing candidate in vitro was tested in vivo in an ovariectomy-induced model of osteoporosis, but an effect on bone loss could not be detected. Based on their powerful antiresorptive activity in vitro, resveratrol analogues might be attractive modulators of bone remodeling. However, further studies are required to establish their efficacy in vivo.