Chemical Synthesis and Immunological Properties of Oligosaccharides Derived from the Vegetative Cell Wall of Bacillus anthracis

• Published in: Chembiochem : a European journal of chemical biology Vol. 9; no. 11; pp. 1716 - 1720
• Main Authors: Vasan, Mahalakshmi, Rauvolfova, Jana, Wolfert, Margreet A, Leoff, Christine, Kannenberg, Elmar L, Quinn, Conrad P, Carlson, Russell W, Boons, Geert-Jan
• Format: Journal Article
• Language: English
• Published: Weinheim Wiley-VCH Verlag 21-07-2008; WILEY-VCH Verlag; WILEY‐VCH Verlag
• Subjects: Animals; Anthrax Vaccines - chemical synthesis; Anthrax Vaccines - immunology; Anthrax Vaccines - metabolism; Antibodies - immunology; Bacillus anthracis; Bacillus anthracis - cytology; Bacillus anthracis - immunology; Bacillus anthracis - metabolism; Cattle; Cell Wall - chemistry; Cell Wall - immunology; Enzyme-Linked Immunosorbent Assay; glycoconjugates; Hemocyanins - metabolism; oligosaccharides; Oligosaccharides - chemical synthesis; Oligosaccharides - immunology; Oligosaccharides - metabolism; protein conjugation; Rabbits; Spores, Bacterial - immunology; Spores, Bacterial - metabolism; vaccines
• ISSN: 1439-4227; 1439-7633
• DOI: 10.1002/cbic.200800210

Sweet relief: Sera from rabbits exposed to Bacillus anthracis Sterne 34F2 spores or immunized with B. anthracis polysaccharide conjugated to keyhole limpet hemocyanin were found to contain antibodies that recognized isolated polysaccharide (shown) and two synthetic trisaccharides. This provides proof‐of‐concept towards the development of vegetative and spore‐specific reagents for detection and targeting of nonprotein structures of B. anthracis.