Molecular cloning of bovine class I MHC cDNA

• Published in: The Journal of immunology (1950) Vol. 141; no. 2; pp. 642 - 651
• Main Authors: Ennis, PD, Jackson, AP, Parham, P
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Am Assoc Immnol 15-07-1988; American Association of Immunologists
• Subjects: Amino Acid Sequence; Animals; Antigens; Base Sequence; beta 2-Microglobulin - genetics; Biological and medical sciences; Cattle; Cell Membrane - metabolism; Cloning, Molecular; Cross Reactions; DNA - isolation & purification; Female; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Genes, MHC Class I; Histocompatibility antigens (hla, h-2 and other systems); Histocompatibility Antigens - genetics; Histocompatibility Antigens - immunology; Histocompatibility Antigens - isolation & purification; HLA Antigens - immunology; Humans; Immunoglobulin Heavy Chains - genetics; Mice; Molecular immunology; Molecular Sequence Data; Protein Sorting Signals - genetics; Protein Sorting Signals - isolation & purification; Sequence Homology, Nucleic Acid; Bovine; Nucleotide sequence; Major histocompatibility system; Homology; B-Lymphocyte; Vertebrata; Immunogenetics; Mammalia; Cell line; Artiodactyla; Molecular cloning; Ungulata; Class I histocompatibility antigen; Comparative study; Aminoacid sequence
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.141.2.642

Two cDNA cloned from a Hereford cow B cell line (BL-3) have allowed the determination of the complete coding region for two class I molecules encoded by the bovine MHC (BoLA). The predicted protein sequences have all the features expected of expressed class I molecules that present peptide Ag to cytotoxic T cells. Comparison with class I molecules from other species strongly suggests these cDNA are derived from different genes and provides evidence for the existence of a second expressed class I BoLA locus. The BoLA proteins show greater similarity to HLA than to H-2 molecules, correlating with the cross-reactions of W6/32 and other murine anti-HLA-A,B,C mAb with BoLA molecules. The basis for the W6/32 epitope and the preferential association of H-2 class I H chains with bovine beta 2-m is examined.