Patch clamp recordings of hair cells isolated from zebrafish auditory and vestibular end organs

• Published in: Neuroscience Vol. 248; pp. 79 - 87
• Main Authors: Haden, M, Einarsson, R, Yazejian, B
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Ltd 17-09-2013; Elsevier
• Subjects: Animals; Biological and medical sciences; Ca2+ channels; Calcium Channels - physiology; Danio rerio; Ear and associated structures. Auditory pathways and centers. Hearing. Vocal organ. Phonation. Sound production. Echolocation; electrophysiology; Freshwater; Fundamental and applied biological sciences. Psychology; Hair Cells, Auditory - physiology; Hair Cells, Vestibular - physiology; inner ear; K+ channels; lagena; Membrane Potentials; Motor control and motor pathways. Reflexes. Control centers of vegetative functions. Vestibular system and equilibration; Neurology; Potassium Channels - physiology; Saccule and Utricle - physiology; utricle; Vertebrates: nervous system and sense organs; Zebrafish; 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; utricle; ethylene glycol bis(β-aminoethylether)- N, N′-tetraacetic acid; NZR; Ca 2+ channels; EGTA; BSA; K + channels; electrophysiology; lagena; HEPES; Normal Zebrafish Ringer’s; inner ear; bovine serum albumin; Ca2+ channels; K+ channels; Calcium; Electrophysiology; Ciliated cell; Patch clamp method; Ionic channel; K; Inner ear; Organ of hearing; Lagena; Vertebrata; Brachydanio rerio; channels; Pisces; Vestibular system; Potassium; Utricle; Ca; Ca(2+) channels; K(+) channels
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/j.neuroscience.2013.05.062

Highlights • Patch clamp recordings were made on zebrafish inner ear hair cells. • Cells displayed substantial diversity in their constellation of K+ channel types. • Ca2+ channels were identified as L-type. • Current injection led to regenerative behavior in a majority of cells.