Analysis of ammonia-oxidizing bacteria populations in acid forest soil during conditions of moisture limitation

• Published in: Letters in applied microbiology Vol. 30; no. 1; pp. 14 - 18
• Main Authors: Hastings, R.C, Butler, C, Singleton, I, Saunders, J.R, McCarthy, A.J
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 2000; Blackwell Science
• Subjects: acid soils; Ammonia - metabolism; Animal, plant and microbial ecology; Biological and medical sciences; Bradyrhizobiaceae - growth & development; Bradyrhizobiaceae - isolation & purification; Bradyrhizobiaceae - metabolism; Colony Count, Microbial; DNA, Bacterial - analysis; DNA, Ribosomal - analysis; Electrophoresis, Agar Gel; forest soils; Fundamental and applied biological sciences. Psychology; Microbial ecology; Nitrosomonas; Nitrosomonas - growth & development; Nitrosomonas - isolation & purification; Nitrosomonas - metabolism; Nitrosospira; plate count; Polymerase Chain Reaction; RNA, Ribosomal, 16S - analysis; Soil; Soil - analysis; soil bacteria; Soil Microbiology; soil water; Water - analysis; Forests; Acid soil; Nitrobacteraceae; Nitrification; Nitrosomonas; Bacteria; Environmental factor; Nitrosospira; Population structure; Drought
• ISSN: 0266-8254; 1472-765X; 1365-2673
• DOI: 10.1046/j.1472-765x.2000.00630.x

R.C. HASTINGS, C. BUTLER, I. SINGLETON, J.R. SAUNDERS and A.J. McCARTHY.2000.Ammonia‐oxidizer numbers decreased under conditions of moisture limitation in litter, fermentation and humus layers of forest soil in the field, but the extent of regrowth after rehydration varied between layers. Nitrosospira 16S rRNA genes were amplified from all layers, regardless of moisture content or soil pH which varied between 4·1 and 5·2. Nitrosomonas spp. were detected less often, but appeared to exhibit more rapid recovery than the Nitrosospira spp. when drought conditions were relieved by rainfall.