Immunohistochemical profile of stromal constituents and lymphoid cells over the course of wound healing in murine model

• Published in: Acta cirurgica brasileira Vol. 29; no. 9; pp. 596 - 602
• Main Authors: Almeida, Bernadeth Moda de, Nascimento, Marismar Fernandes do, Pereira-Filho, Rose Nely, Melo, Genecy Calado de, Santos, José Cleveilton dos, Oliveira, Clauberto Rodrigues de, Gomes, Margarete Zanardo, Lima, Sônia Oliveira, Albuquerque-Júnior, Ricardo Luiz Cavalcanti de
• Format: Journal Article
• Language: English
• Published: Brazil Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 01-09-2014
• Subjects: Actins - metabolism; Animals; Antigens, CD - metabolism; Antigens, CD20 - metabolism; CD3 Complex - metabolism; Collagen Type I - metabolism; Disease Models, Animal; Histological Techniques; Immunohistochemistry; Lymphocytes - pathology; Male; Myofibroblasts - pathology; Platelet Endothelial Cell Adhesion Molecule-1 - metabolism; Rats, Wistar; Skin - injuries; Skin - metabolism; Stromal Cells - metabolism; Stromal Cells - pathology; Time Factors; Wound Healing; Wound Healing - physiology
• ISSN: 0102-8650; 1678-2674; 0102-8650
• DOI: 10.1590/s0102-8650201400150007

To assess the evolution profile of the immunohistochemical expression of stromal constituents over the time-course of wound healing in a murine model.
Surgical wounds were performed in the back of 24 Wistar rats. After three, seven, 14 and 21 days, six rats were euthanized and the wounded histologically processed to assess the immunohistochemical expression of CD3, CD20, CD31, α-SMA and type-I collagen. Non-injured skin samples (NSS) were used as control. Data were subjected to statistical analysis using ANOVA and Tukey test.
The mean of CD3 and CD20 positive cells in the wounds was significantly higher than in NSS at seven and 14 days (p<0.001). The blood vessels content was significantly lower than in NSS (p<0.05) at three days, but increased at seven and 14 days (p<0.01). The mean of α-SMA positive cells at seven, 14 and 21 days was higher than in NSS (p<0.05). The relative content of type I collagen increased from three to 21 days, but remained lower than in NSS (p<0.05).
Lymphoid cells, myofibroblasts and microvessels contents varied over the time-course of wound healing, with peak at seven days and progressive reduction until 21 days. The type I collagen content increased over time.