Crimean–Congo hemorrhagic fever virus genome L RNA segment and encoded protein

• Published in: Virology (New York, N.Y.) Vol. 321; no. 1; pp. 29 - 35
• Main Authors: Honig, Jessica E, Osborne, Jane C, Nichol, Stuart T
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 30-03-2004
• Subjects: Amino Acid Motifs - genetics; Amino Acid Sequence; Congo; Crimean; Crimean-Congo hemorrhagic fever virus; Deubiquitination; DNA-Directed RNA Polymerases - genetics; Dugbe; Genome, Viral; Hemorrhagic; Hemorrhagic Fever Virus, Crimean-Congo - chemistry; Hemorrhagic Fever Virus, Crimean-Congo - enzymology; Hemorrhagic Fever Virus, Crimean-Congo - genetics; Molecular Sequence Data; Nairovirus; OTU; Polymerase; Reverse Transcriptase Polymerase Chain Reaction; Sequence Alignment; Virus; Nairovirus; Virus; Polymerase; OTU; Deubiquitination; Crimean; L; Dugbe; Congo; Hemorrhagic
• ISSN: 0042-6822; 1096-0341
• DOI: 10.1016/j.virol.2003.09.042

Sequence analysis of the L RNA genome segment and predicted encoded L polymerase protein of Crimean–Congo hemorrhagic fever (CCHF) virus (genus
Nairovirus, family
Bunyaviridae) demonstrates that they are approximately twice the size of those found in viruses of other bunyavirus genera. The CCHF virus L segment and encoded protein (12164 nucleotides and 3944 amino acids, respectively) are similar in size and sequence to those of the nairovirus Dugbe virus (12255/62% and 4036/62% nucleotide and amino acid length/identity, respectively). The identification of an ovarian tumor (OTU)-like protease motif in the L protein amino termini of the nairoviruses Dugbe, CCHF, and Nairobi sheep disease (NSD) indicates these proteins are members of the recently described OTU-like protease family and suggests that these large proteins may be polyproteins that are autoproteolytically cleaved or involved in deubiquitination.