Investigation of isotope dilution mass spectrometric (ID-MS) method to determine niacin in infant formula, breakfast cereals and multivitamins

• Published in: Food chemistry Vol. 138; no. 2-3; pp. 1109 - 1115
• Main Authors: Shin, Hyunju, Kim, Byungjoo, Lee, Joonhee
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01-06-2013; Elsevier
• Subjects: Biological and medical sciences; Breakfast cereals; Cereal and baking product industries; Dietary Supplements - analysis; Drug Contamination; Edible Grain - chemistry; Food Contamination - analysis; Food industries; Fundamental and applied biological sciences. Psychology; ID-LC/MS; Infant formula; Infant Formula - chemistry; Mass Spectrometry - methods; Method validation; Multivitamin; Niacin; Niacin - analysis; ID-LC/MS; Multivitamin; Method validation; Infant formula; Breakfast cereals; Niacin; Validation; Nicotinic acid; Isotope dilution; Liquid chromatography; Method; Mass; Cereal product
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2012.11.046

► ID-LC/MS method for niacin analysis in food matrix sample. ► Different sample preparation depending on existing forms of niacin in samples. ► Method validation by reference materials and repeatability/reproducibility check. ► Application of developed method on commercial samples.
An isotope dilution LC/mass spectrometric (ID-LC/MS) method was developed as a candidate reference method for the accurate determination of niacin in infant formula, breakfast cereals and multivitamin. After spiking nicotinamide-d4 as an internal standard, infant formula and breakfast cereal samples were hydrolysed under alkaline condition. Samples were then analysed in SRM mode to detect nicotinic acid and nicotinic acid-d4 at m/z 124→80 and 127→84, respectively. In the case of multivitamin sample that contains mainly free nicotinamide, LC/MS monitored nicotinamide and nicotinamide-d4 at their SRM channels of m/z 123→80 and m/z 127→84, respectively, after simple extraction. The repeatability and reproducibility were tested for the validation of the developed ID/LC–MS method. Additionally, the developed analytical method was applied to determine total niacin contents in homogenised infant formula, homogenised multivitamin, and commercially available products including different types of infant formula, breakfast cereals, and multivitamin tablets.