Targeting Cre Recombinase to Specific Neuron Populations with Bacterial Artificial Chromosome Constructs

Transgenic mouse lines are characterized with Cre recombinase driven by promoters of CNS-specific genes using bacterial artificial chromosome (BAC) constructs. BAC-Cre constructs for 10 genes ( Chat , Th , Slc6a4 , Slc6a2 , Etv1 , Ntsr1 , Drd2 , Drd1 , Pcp2 , and Cmtm5 ) produced 14 lines with Cre e...

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Bibliographic Details
Published in:	The Journal of neuroscience Vol. 27; no. 37; pp. 9817 - 9823
Main Authors:	Gong, Shiaoching, Doughty, Martin, Harbaugh, Carroll R, Cummins, Alexander, Hatten, Mary E, Heintz, Nathaniel, Gerfen, Charles R
Format:	Journal Article
Language:	English
Published:	United States Soc Neuroscience 12-09-2007 Society for Neuroscience
Subjects:	Animals Chromosomes, Artificial, Bacterial - enzymology Chromosomes, Artificial, Bacterial - genetics Gene Expression Regulation, Enzymologic - physiology Gene Targeting - methods Genetic Vectors Integrases - biosynthesis Integrases - genetics Integrases - metabolism Mice Mice, Transgenic Nerve Net - enzymology Neurons - enzymology Toolbox
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Summary:	Transgenic mouse lines are characterized with Cre recombinase driven by promoters of CNS-specific genes using bacterial artificial chromosome (BAC) constructs. BAC-Cre constructs for 10 genes ( Chat , Th , Slc6a4 , Slc6a2 , Etv1 , Ntsr1 , Drd2 , Drd1 , Pcp2 , and Cmtm5 ) produced 14 lines with Cre expression in specific neuronal and glial populations in the brain. These Cre driver lines add functional utility to the >500 BAC-EGFP (enhanced green fluorescent protein) transgenic mouse lines that are part of the Gene Expression Nervous System Atlas Project.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0270-6474 1529-2401
DOI:	10.1523/JNEUROSCI.2707-07.2007