Evaluation of a Hydrogel-Based Diagnostic Approach for the Point-of-Care Based Detection of Neisseria gonorrhoeae

• Published in: Antibiotics (Basel) Vol. 7; no. 3; p. 70
• Main Authors: Perera, Sumudu R, Taheri, Ali, Khan, Nurul H, Parti, Rajinder P, Stefura, Stephanie, Skiba, Pauline, Acker, Jason P, Martin, Irene, Kusalik, Anthony, Dillon, Jo-Anne R
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 04-08-2018; MDPI
• Subjects: Antimicrobial agents; Chlamydia; Condoms; Deoxyribonucleic acid; Diagnostic systems; Diagnostic tests; DNA; Females; Gonorrhea; hydrogel; Hydrogels; Infections; Laboratories; molecular diagnosis; Neisseria; Neisseria gonorrhoeae; Polymerase chain reaction; Primers; Public health; RT-PCR; Sensitivity; Sensitivity analysis; Canada; Neisseria gonorrhoeae; hydrogel; RT-PCR; molecular diagnosis
• ISSN: 2079-6382; 2079-6382
• DOI: 10.3390/antibiotics7030070

Eleven primer pairs were developed for the identification of
. The sensitivity and specificity of these primers were evaluated by Real Time (RT)-PCR melt curve analyses with DNA from 145
isolates and 40 other
or non-
species. Three primer pairs were further evaluated in a hydrogel-based RT-PCR detection platform, using DNA extracted from 50
cultures. We observed 100% sensitivity and specificity in the hydrogel assay, confirming its potential as a point-of-care test (POCT) for
diagnosis.