Analysis of Bile Acids and Cholesterol by Open Tubular Glass Capillary-Selected Ion Monitoring

Analysis of Bile Acids and Cholesterol by Open Tubular Glass Capillary-Selected Ion Monitoring

A method for the baseline separation of the dimethylethylsilyl (DMES) ether derivatives of bile acid ethyl esters and cholesterol was developed by using selected ion monitoring (SIM) with open tublar glass capillary column coated with SE-30. The DEMS ether derivatives of bile acid ethyl esters were...

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Bibliographic Details
Published in:Chemical & pharmaceutical bulletin Vol. 26; no. 9; pp. 2922 - 2923
Main Authors: NISHIKAWA, YUKO, YAMASHITA, KOUWA, ISHIBASHI, MASATAKA, MIYAZAKI, HIROSHI
Format: Journal Article
Language:English
Published: The Pharmaceutical Society of Japan 01-01-1978
Subjects:
WCOT column
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Summary:A method for the baseline separation of the dimethylethylsilyl (DMES) ether derivatives of bile acid ethyl esters and cholesterol was developed by using selected ion monitoring (SIM) with open tublar glass capillary column coated with SE-30. The DEMS ether derivatives of bile acid ethyl esters were eluted in the regular order according to the number of hydroxyl group in the molecule. The detection limit of the DMES ether derivative of lithocholic acid ethyl ester was 5 pg with S/N ratio at 5 : 1.
ISSN:0009-2363
1347-5223
DOI:10.1248/cpb.26.2922