Antitrypanosomal alkaloids from the marine bacterium Bacillus pumilus

• Published in: Molecules (Basel, Switzerland) Vol. 17; no. 9; pp. 11146 - 11155
• Main Authors: Martínez-Luis, Sergio, Gómez, José Félix, Spadafora, Carmenza, Guzmán, Héctor M, Gutiérrez, Marcelino
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 18-09-2012; MDPI
• Subjects: Alkaloids - chemistry; Alkaloids - isolation & purification; Alkaloids - metabolism; Alkaloids - pharmacology; Animals; Anthozoa - microbiology; Bacillus - isolation & purification; Bacillus - metabolism; Bacillus pumilus; Bacteria; Cell Line; Cercopithecus aethiops; Chagas disease; Humans; indole alkaloids; Infections; Leishmania donovani - drug effects; Metabolites; Parasitic Sensitivity Tests; Plasmodium falciparum - drug effects; Protozoa; R&D; Research & development; Trypanocidal Agents - chemistry; Trypanocidal Agents - isolation & purification; Trypanocidal Agents - pharmacology; Trypanosoma cruzi; Trypanosoma cruzi - drug effects; Vero Cells; Panama
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules170911146

Fractionation of the ethyl acetate extract of the marine bacterium Bacillus pumilus isolated from the black coral Antipathes sp. led to the isolation of five compounds: cyclo-(L-Leu-L-Pro) (1), 3-hydroxyacetylindole (2), N-acetyl-β-oxotryptamine (3), cyclo-(L-Phe-L-Pro) (4), and 3-formylindole (5). The structures of compounds 1-5 were established by spectroscopic analyses, including HRESITOF-MS and NMR (1H, 13C, HSQC, HMBC and COSY). Compounds 2, 3 and 5 caused the inhibition on the growth of Trypanosoma cruzi (T. cruzi), with IC50 values of 20.6, 19.4 and 26.9 μM, respectively, with moderate cytotoxicity against Vero cells. Compounds 1-5 were found to be inactive when tested against Plasmodium falciparum and Leishmania donovani, therefore showing selectivity against T. cruzi parasites.