Detection of camel pox and vaccinia viruses by polymerase chain reaction

• Published in: Tropical animal health and production Vol. 41; no. 8; pp. 1637 - 1641
• Main Authors: Sheikh Ali, Hanan M, Khalafalla, A. I, Nimir, A. H
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01-12-2009; Springer Netherlands; Springer Nature B.V
• Subjects: Animals; Biomedical and Life Sciences; Cell culture; Chlorocebus aethiops; Chorioallantoic membrane; Cytopathogenic Effect, Viral; Deoxyribonucleic acid; DNA; Life Sciences; Membranes; Neutralization; Neutralization Tests; Orthopoxvirus - isolation & purification; Polymerase chain reaction; Polymerase Chain Reaction - methods; Tissue culture; Vaccinia virus; Vaccinia virus - isolation & purification; Vero Cells; Veterinary Medicine/Veterinary Science; Viruses; Zoology; Vaccinia virus; Camel pox virus; PCR
• ISSN: 0049-4747; 1573-7438
• DOI: 10.1007/s11250-009-9359-y

PCR following two methods of DNA extraction was used to confirm the growth of camel pox virus (CPV) and vaccinia virus in cell culture and chorioallantoic membrane. Results were compared with the commonly used neutralization test. The first method of DNA extraction was accomplished by using viral DNA in tissue culture supernatant and Chorioallantoic membrane, which was released by initial heating for 15 min at 99°C followed by ordinary PCR. In the second method DNA was extracted by using DNA Isolation Kit from tissue culture supernatant and used as a template. Rapid identification and differentiation of CPV and Vaccinia virus were achieved by PCR and this assay proved to be fast and feasible, and can be an alternative to orthodox serological methods.