Towards engineered yeast as production platform for capsaicinoids

• Published in: Biotechnology advances Vol. 59; no. 107989; p. 107989
• Main Authors: Muratovska, Nina, Silva, Paulo, Pozdniakova, Tatiana, Pereira, Humberto, Grey, Carl, Johansson, Björn, Carlquist, Magnus
• Format: Journal Article
• Language: English
• Published: England Elsevier Inc 01-10-2022; Elsevier 1; Elsevier Science Ltd
• Subjects: Acyl-CoA; Baking yeast; Bioengineering; Biologi; Biological Sciences; Biosynthesis; Capsaicin synthase; Capsaicinoids; Capsicum; Chemical synthesis; Ciências Biológicas; Ciências Naturais; Engineering and Technology; Fatty acids; Fermentation; Industrial Biotechnology; Industriell bioteknik; Ion channels; Microbiology; Microorganisms; Mikrobiologi; Natural Sciences; Naturvetenskap; Peppers; Receptors; Saccharomyces cerevisiae; State-of-the-art reviews; Teknik; TRPV1; Vanillin; Vanillylamine; NAT; ALDs; DH; Acyl-CoA; KDH; AlDH; MPT; FADS3; FADS2; 8MNA; FADS4; AKRs; 3DSD; MDR; 4CL; MCFAs; SDR; ATs; BCFA; Fermentation; HCBT; Fatty acids; ER; AAE1; AT; OMT; FA; KAS; Saccharomyces cerevisiae; SAM; BCAA; IREDs; Vanillylamine; ACAR; PDC; Capsaicinoids; PPT; PLP; PDH; Cv-ATA; NAT-CL; FASII; AmDHs; KR; Capsaicin synthase; HQT; TRPV1; ATA; BCKDH; TE; FASI; ACS; PAC; FASN; FAS; Vanillin; Capsicum; NRPSs; ABSs
• ISSN: 0734-9750; 1873-1899
• DOI: 10.1016/j.biotechadv.2022.107989

Capsaicinoids are bioactive alkaloids produced by the chili pepper fruit and are known to be the most potent agonists of the human pain receptor TRPV1 (Transient Receptor Potential Cation Channel Subfamily V Member 1). They are currently produced by extraction from chili pepper fruit or by chemical synthesis. Transfer of the biosynthetic route to a microbial host could enable more efficient capsaicinoid production by fermentation and may also enable the use of synthetic biology to create a diversity of new compounds with potentially improved properties. This review summarises the current state of the art on the biosynthesis of capsaicinoid precursors in baker's yeast, Saccharomyces cerevisiae, and discusses bioengineering strategies for achieving total synthesis from sugar.