Development of Dual Ion-selective Electrodes in Double-Barrel Glass Pipette at One Micrometer for Simultaneous Measurement of Sodium and Potassium Ions

• Published in: E-journal of surface science and nanotechnology Vol. 21; no. 1; pp. 17 - 23
• Main Authors: Takami, Tomohide, Akutsu, Yusuke, Kaneko, Naoki, Yoneda, Rio, Magara, Hideyuki, Ogawa, Shuichi, Abukawa, Tadashi
• Format: Journal Article
• Language: English
• Published: Tokyo The Japan Society of Vacuum and Surface Science 27-10-2022; Japan Science and Technology Agency
• Subjects: Crown ethers; Double-barrel pipette; Electrodes; Ion-selective electrode; Micropipette; Potassium; Potassium ion; Selectivity; Sodium; Sodium ion; Vinyl chloride
• ISSN: 1348-0391; 1348-0391
• DOI: 10.1380/ejssnt.2023-003

We have developed the method of preparing sodium and potassium ion-selective electrodes in double-barrel glass pipettes at the size of 1 μm. Stable and precise fabrication parameters were found for producing a double-barrel 1 μm pipette, and the produced double-barrel micropipettes were used in this study. The ionophores comprising of crown ethers of 12-crown-4 for sodium ions and 15-crown-5 for potassium ions were respectively doped in poly(vinyl chloride) films in the divided double-barrel glass pipette. The obtained selectivity coefficients and selectivity ratios are compared with the previous studies. The developed ion-selective dual-micropipettes can be applied for local ion-selective measurements such as living cells.