Evidence for hydrophobic catalysis of DNA strand exchange

The catalytic role of hydrophobic co-solutes in DNA strand exchange is demonstrated by FRET kinetics. Two mechanisms that contribute to this are base stacking destabilisation and nucleation-promoted DNA strand invasion. We propose that hydrophobic catalysis is involved in the strand-exchange activit...

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Bibliographic Details
Published in:	Chemical communications (Cambridge, England) Vol. 51; no. 34; p. 7390
Main Authors:	Feng, B, Westerlund, F, Nordén, B
Format:	Journal Article
Language:	English
Published:	England 01-01-2015
Subjects:	Biocatalysis DNA - chemistry Hydrophobic and Hydrophilic Interactions Nucleic Acid Denaturation Polyethylene Glycols - chemistry Transition Temperature
Online Access:	Get more information
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Summary:	The catalytic role of hydrophobic co-solutes in DNA strand exchange is demonstrated by FRET kinetics. Two mechanisms that contribute to this are base stacking destabilisation and nucleation-promoted DNA strand invasion. We propose that hydrophobic catalysis is involved in the strand-exchange activity of recombination enzymes.
ISSN:	1364-548X
DOI:	10.1039/c5cc01515d