Growth characteristic of Camel pox and Vaccinia viruses in embryonated eggs and cell culture

• Published in: Tropical animal health and production Vol. 41; no. 3; pp. 393 - 396
• Main Authors: Sheikh Ali, Hanan M, Nimir, Abdel Hafeez H, Khalafalla, Abdelmalik I
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01-03-2009; Springer Netherlands; Springer Nature B.V
• Subjects: Animals; Biomedical and Life Sciences; CAM; Camel pox virus; Camelus; Cell culture; Cercopithecus aethiops; Chick Embryo; Chorioallantoic membrane; Chorioallantoic Membrane - virology; CPE; Eggs; Eggs - virology; Inoculation; Life Sciences; Membranes - virology; Original Paper; Poxviridae - growth & development; Rounding; Syncytia; Vaccinia virus; Vaccinia virus - growth & development; Vero cells; Vero Cells - virology; Veterinary Medicine/Veterinary Science; Viruses; Zoology; Vaccinia virus; CPE; Camel pox virus; CAM; Vero cells
• ISSN: 0049-4747; 1573-7438
• DOI: 10.1007/s11250-008-9202-x

Camel pox viruses isolated in Sudan and VD45 (African camel pox strain) and Vaccinia virus (Elstree strain) were used for inoculation of chorioallantoic membrane (CAM) of embryonated eggs (EE) and cell culture (CC). In EE Lesions were seen as pocks ranging in size from 1 to 1.5 mm in diameter, and they increase in size with serial passage and taking opaque- white and opaque- yellow colors. When propagated in Vero cells, these viruses gave clear CPE, characterized by rounding of cells, plaque formation, syncytia and detachment of cells from the glass.