Evidence for two serotype G3 subtypes among equine rotaviruses

• Published in: Journal of Clinical Microbiology Vol. 30; no. 2; pp. 485 - 491
• Main Authors: Browning, G.F, Chalmers, R.M, Fitzgerald, T.A, Snodgrass, D.R
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01-02-1992
• Subjects: Animals; Antibodies, Monoclonal; Antigens, Viral; Biological and medical sciences; Capsid - immunology; Capsid Proteins; characterization; Enzyme-Linked Immunosorbent Assay; Epidemiologic Methods; epidemiology; Epitopes; Fundamental and applied biological sciences. Psychology; genetic variation; Horse Diseases - epidemiology; Horse Diseases - microbiology; Horses; isolation; Microbiology; Neutralization Tests; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains; Rotavirus; Rotavirus - classification; Rotavirus - immunology; Rotavirus - isolation & purification; Rotavirus Infections - epidemiology; Rotavirus Infections - microbiology; Rotavirus Infections - veterinary; Serotyping; strains; Virology; Northern Ireland; United Kingdom; North America; Virus; Antigenicity; Isolation; Monoclonal antibody; Duovirus; Reoviridae; Serotyping; Strain
• ISSN: 0095-1137; 1098-660X
• DOI: 10.1128/jcm.30.2.485-491.1992

Ten cultivable equine rotavirus isolates, two of North American, six of British, and two of Irish origin, were compared with standard rotavirus strains and with each other by cross neutralization, neutralization with a panel of monoclonal antibodies (MAbs), hybridization to a simian rotavirus (SA-11) VP7 gene probe, and reaction with rotavirus subgrouping and serotyping MAbs in enzyme-linked immunosorbent assays. Six isolates, two of which had previously been serotyped as G3 by other workers, were found to be serotype G3; one was confirmed to be G5, and three were not related to serotypes G1 to G10. The serotype G3 strains were divisible into two subtypes, G3A and G3B, on the basis of cross neutralization. This division was also apparent in reactions with neutralizing Vp7-specific MAbs and in the liquid hybridization assay. Two of the isolates were not bound by either subgroup MAb, six were bound by both subgroup I and II MAbs, and two were bound by only the subgroup I MAb. The assays used in this characterization provide a range of epidemiological information for use in future field investigations.