A rare case of lung coinfection by Streptomyces cinereoruber and Haemophilus influenzae in a patient with severe chronic obstructive pulmonary disease: characterization at species level using molecular techniques

• Published in: Diagnostic microbiology and infectious disease Vol. 60; no. 3; pp. 307 - 311
• Main Authors: Manteca, Angel, Pelaez, Ana Isabel, del Mar Garcia-Suarez, Maria, Hidalgo, Elisa, del Busto, Benigno, Mendez, Francisco J
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01-03-2008; Elsevier
• Subjects: 16S rDNA; Aged; Anti-Bacterial Agents - therapeutic use; Anti-Inflammatory Agents - therapeutic use; Bacterial Typing Techniques; Bacteriology; Biological and medical sciences; Chronic obstructive pulmonary disease; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Fundamental and applied biological sciences. Psychology; Haemophilus Infections - complications; Haemophilus Infections - drug therapy; Haemophilus influenzae; Haemophilus influenzae - genetics; Haemophilus influenzae - isolation & purification; Humans; Infectious Disease; Internal Medicine; Male; Microbiology; Miscellaneous; Polymorphism, Restriction Fragment Length; Prednisone - therapeutic use; Pulmonary Disease, Chronic Obstructive - drug therapy; Pulmonary Disease, Chronic Obstructive - microbiology; Radiography, Thoracic; RNA, Ribosomal, 16S - genetics; Streptomyces; Streptomyces - genetics; Streptomyces - isolation & purification; Sulfamethoxazole - therapeutic use; Tomography, X-Ray Computed; Trimethoprim - therapeutic use; Streptomyces; Chronic obstructive pulmonary disease; 16S rDNA; Human; Lung disease; Streptomycetaceae; Mixed infection; Respiratory disease; Lung; Ribosomal DNA; Respiratory system; Haemophilus influenzae; Actinomycetales; Pasteurellaceae; Streptomyces cinereoruber; Bacteria; Bronchus disease; Actinomycetes
• ISSN: 0732-8893; 1879-0070
• DOI: 10.1016/j.diagmicrobio.2007.10.009

Abstract Streptomyces cinereoruber isolated from a patient with severe respiratory failure that could not be identified by culture was correctly identified at the species level by comparative sequence analysis of 16S rDNA and the hypervariable α-region of 16S rDNA. Restriction fragment length polymorphism, morphologic, and biochemical characteristics have confirmed their identity.