PCNA Is Required for Initiation of Recombination-Associated DNA Synthesis by DNA Polymerase δ
Genetic recombination ensures proper chromosome segregation during meiosis and is essential for genome stability and tumor suppression. DNA synthesis after Rad51-mediated DNA strand invasion is a crucial step during recombination. PCNA is known as the processivity clamp for DNA polymerases. Here, we...
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Published in: | Molecular cell Vol. 36; no. 4; pp. 704 - 713 |
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Main Authors: | , , , |
Format: | Journal Article |
Language: | English |
Published: |
United States
Elsevier Inc
25-11-2009
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Subjects: | |
Online Access: | Get full text |
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Summary: | Genetic recombination ensures proper chromosome segregation during meiosis and is essential for genome stability and tumor suppression. DNA synthesis after Rad51-mediated DNA strand invasion is a crucial step during recombination. PCNA is known as the processivity clamp for DNA polymerases. Here, we report the surprising observation that PCNA is specifically required to initiate recombination-associated DNA synthesis in the extension of the 3′ end of the invading strand in a D loop. We show using a reconstituted system of yeast Rad51, Rad54, RPA, PCNA, RFC, and DNA polymerase δ that loading of PCNA by RFC targets DNA polymerase δ to the D loop formed by Rad51 protein, allowing efficient utilization of the invading 3′ end and processive DNA synthesis. We conclude that PCNA has a specific role in the initiation of recombination-associated DNA synthesis and that DNA polymerase δ promotes recombination-associated DNA synthesis. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1 Present address: Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston MA 02115. |
ISSN: | 1097-2765 1097-4164 |
DOI: | 10.1016/j.molcel.2009.09.036 |