Comparison of Hand-Held Test Kits, Immunofluorescence Microscopy, Enzyme-Linked Immunosorbent Assay, and Flow Cytometric Analysis for Rapid Presumptive Identification of Yersinia pestis

An in-house immunochromatographic test, Plague BioThreat Alert test strips, ABICAP columns, enzyme-linked immunosorbent assay, flow cytometry, and immunofluorescence microscopy were compared for the detection of the fraction 1 capsular antigen of Yersinia pestis, using spiked buffer and clinical spe...

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Bibliographic Details
Published in:	Journal of Clinical Microbiology Vol. 45; no. 10; pp. 3404 - 3407
Main Authors:	Tomaso, H, Thullier, P, Seibold, E, Guglielmo, V, Buckendahl, A, Rahalison, L, Neubauer, H, Scholz, H.C, Splettstoesser, W.D
Format:	Journal Article
Language:	English
Published:	Washington, DC American Society for Microbiology 01-10-2007
Subjects:	Bacterial Proteins - analysis Bacteriology Biological and medical sciences Enzyme-Linked Immunosorbent Assay - methods Flow Cytometry - methods Fundamental and applied biological sciences. Psychology Microbiology Microscopy, Fluorescence Miscellaneous Reagent Kits, Diagnostic Yersinia pestis Yersinia pestis - isolation & purification Flow cytometry Microscopy Bacteria Identification Immunofluorescence ELISA assay Enterobacteriaceae Yersinia pestis
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Summary:	An in-house immunochromatographic test, Plague BioThreat Alert test strips, ABICAP columns, enzyme-linked immunosorbent assay, flow cytometry, and immunofluorescence microscopy were compared for the detection of the fraction 1 capsular antigen of Yersinia pestis, using spiked buffer and clinical specimens. Hand-held test kits proved to be excellent benchtop tools.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Corresponding author. Mailing address: Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, 80937 Munich, Germany. Phone: 49-8931683808. Fax: 49-8931683292. E-mail: herberttomaso@bundeswehr.org
ISSN:	0095-1137 1098-660X 1098-5530
DOI:	10.1128/JCM.00458-07