A growth inhibitory protein secreted by human diploid fibroblasts. Partial purification and characterization

• Published in: The Journal of biological chemistry Vol. 262; no. 5; pp. 2176 - 2179
• Main Authors: Feltham, N., Fahey, D., Knight, E.
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Elsevier Inc 15-02-1987; American Society for Biochemistry and Molecular Biology
• Subjects: Biological and medical sciences; Cell differentiation, maturation, development, hematopoiesis; Cell physiology; Cycloheximide - pharmacology; Dactinomycin - pharmacology; fibroblast-derived growth inhibitor; fibroblasts; Fibroblasts - metabolism; Fundamental and applied biological sciences. Psychology; Glycopeptides - isolation & purification; Humans; man; Molecular and cellular biology; Molecular Weight; Poly I-C - pharmacology; Thymidine - metabolism; Characterization; Human; Proteins; Cell culture; Purification; Growth; Production; Inhibitor; Fibroblast; Biological activity
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1016/S0021-9258(18)61635-7

We have identified and partially purified a growth inhibitor protein secreted by human diploid fibroblast cells. This protein is not secreted constitutively but only after induction with the double stranded hetero duplex polyriboinosinic:polyribocytidylic acid. The growth inhibitory activity has been purified 3,800-fold and has an estimated molecular mass of 12,000 daltons. The protein will inhibit the growth in culture of human diploid fibroblast cells, human cells derived from tumors, and mouse L cells. Although interferon-beta is secreted with the growth inhibitory protein, the partially purified growth inhibitory protein has no antiviral activity, and its activity is not neutralized by antibodies to interferon-alpha, interferon-beta, and interferon-gamma. We believe this growth inhibitory activity to reside in a newly defined protein and have named it fibroblast-derived growth inhibitor.