Comparative Pharmacokinetics of Gallic Acid, Protocatechuic Acid, and Quercitrin in Normal and Pyelonephritis Rats after Oral Administration of a Polygonum capitatum Extract

• Published in: Molecules (Basel, Switzerland) Vol. 24; no. 21; p. 3873
• Main Authors: Huang, Yong, Zhou, Zuying, Yang, Wu, Gong, Zipeng, Li, Yueting, Chen, Siying, Wang, Yonglin, Wang, Aimin, Lan, Yanyu, Liu, Ting, Zheng, Lin
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 27-10-2019; MDPI
• Subjects: Acids; Animals; Aquatic plants; Blood; Chinese medicine; Constituents; Disease; Drug metabolism; Drugs, Chinese Herbal - therapeutic use; Enzymes; Female; Gallic acid; Gallic Acid - pharmacokinetics; Hydroxybenzoates - pharmacokinetics; Inflammation; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Medical research; Metabolism; Metabolites; Oral administration; Parameter estimation; Pharmacokinetics; Pharmacology; Physiology; Plant Extracts - therapeutic use; Plasma; Polygonum - chemistry; Polygonum capitatum; polygonum capitatum extract; Protocatechuic acid; Pyelonephritis; Pyelonephritis - drug therapy; Pyelonephritis - metabolism; Quercetin - analogs & derivatives; Quercetin - pharmacokinetics; quercitrin; Rats; Rats, Sprague-Dawley; uplc-ms/ms; Urinary tract; Urinary tract diseases; Urinary tract infections; Urogenital system; China; pyelonephritis; Polygonum capitatum extract; quercitrin; pharmacokinetics; UPLC-MS/MS; gallic acid; protocatechuic acid
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules24213873

Buch.-Ham. ex D. Don is traditionally used by Hmong for the treatment of urinary tract infections and pyelonephritis. Information regarding the pharmacokinetic behavior of the extract in the condition of pyelonephritis is lacking. In the present study, we aimed to compare the pharmacokinetic properties of gallic acid (GA), protocatechuic acid (PCA), and quercitrin (QR)-the main bioactive constituents in the herb-in normal and pyelonephritis rats. The plasma samples were collected at various time points after administration of a single dose of
extract. The plasma level of GA, PCA, and QR at the designed time points was determined by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) and drug concentration versus time plots were constructed to estimate the pharmacokinetic parameters. The AUC
, AUC
, MRT
, and CL of GA, PCA, and QR in pyelonephritis rats was significantly different from those of the normal rats. The results indicated that the three constituents have higher rate of uptake and slower rate of elimination in the rats with pyelonephritis, suggesting altered rate and extent of drug metabolism.