Cell-Free Supernatant from Lactobacillus and Streptococcus Strains Modulate Mucus Production via Nf-κB/CREB Pathway in Diesel Particle Matter-Stimulated NCI-H292 Airway Epithelial Cells

• Published in: Molecules (Basel, Switzerland) Vol. 28; no. 1; p. 61
• Main Authors: Lee, Ji Yeon, Kang, Chang-Ho
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 21-12-2022; MDPI
• Subjects: airway inflammation; Anti-inflammatory agents; cAMP response element-binding protein; Chronic obstructive pulmonary disease; Cigarettes; Circulatory system; Coronaviruses; COVID-19; Cyclic AMP response element-binding protein; Cyclic AMP Response Element-Binding Protein - metabolism; Cytokines; Cytotoxicity; Epithelial cells; Epithelial Cells - metabolism; Epithelial Cells - pathology; Epithelium; Gene expression; Homeostasis; Humans; Inflammation; Inflammation - prevention & control; Inflammatory diseases; Kinases; Lactobacillus; Lactobacillus - metabolism; Lung diseases; Lungs; Metabolites; Mortality; MUC5B gene; Mucin; Mucins - metabolism; Mucus; Mucus - metabolism; NF-kappa B - metabolism; NF-κB protein; Obstructive lung disease; Particulate matter; Pathogenesis; Phosphorylation; Probiotics; Protein expression; Proteins; Respiratory tract; Respiratory tract diseases; Signal transduction; Streptococcus; Tumor necrosis factor-TNF; airway inflammation; Streptococcus; cAMP response element-binding protein; Lactobacillus
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules28010061

Airway epithelial cells are a major site of airway inflammation and may play an important role in the pathogenesis of chronic obstructive pulmonary disease (COPD). Diesel particulate matter (DPM) is associated with mucus hypersecretion and airway inflammation and has been reported to overexpress airway mucin in the NCI-H292 airway epithelial cells. Therefore, regulation of mucin hypersecretion is essential for developing novel anti-inflammatory agents. This study aimed to investigate the effects of cell-free supernatant (CFS) from
and
on nitro oxide (NO) production in RAW264.7 and proteins associated with mucus production in NCI-H292 cells. We observed that NO production was reduced by CFS from
and
in RAW 264.7, and
,
, and
gene expression was increased by phosphorylation of nuclear factor kappa B (NF-κB) p65 and cAMP response element-binding protein (CREB) in DPM-stimulated NCI-H292 cells. However, CFS from
MG4272, MG4577,
MG4247, and
MG5140 inhibited mRNA expression related to mucus production by downregulating the CREB/NfκB signaling pathway. These results suggest
CFS from
MG4272, MG4577,
MG4247, and
MG5140 can contribute as a strategic candidate to the prevention of airway inflammatory diseases caused by DPM.