miR-374 promotes myocardial hypertrophy by negatively regulating vascular endothelial growth factor receptor-1 signaling

• Published in: BMB reports Vol. 50; no. 4; pp. 208 - 213
• Main Authors: Lee, Jong Sub, Song, Dong Woo, Park, Jei Hyoung, Kim, Jin Ock, Cho, Chunghee, Kim, Do Han
• Format: Journal Article
• Language: English
• Published: Korea (South) Korean Society for Biochemistry and Molecular Biology 01-04-2017; 생화학분자생물학회
• Subjects: 3' Untranslated Regions; Animals; Antagomirs - metabolism; Base Sequence; Calcium-Calmodulin-Dependent Protein Kinase Type 2 - metabolism; Cardiomegaly - genetics; Cardiomegaly - metabolism; Cardiomegaly - pathology; Cells, Cultured; Cyclic GMP-Dependent Protein Kinase Type I - antagonists & inhibitors; Cyclic GMP-Dependent Protein Kinase Type I - genetics; Cyclic GMP-Dependent Protein Kinase Type I - metabolism; Genes, Reporter; MEF2 Transcription Factors - metabolism; MicroRNAs - antagonists & inhibitors; MicroRNAs - genetics; MicroRNAs - metabolism; Myocytes, Cardiac - cytology; Myocytes, Cardiac - metabolism; NFATC Transcription Factors - metabolism; Rats; Rats, Sprague-Dawley; RNA Interference; RNA, Small Interfering - metabolism; Sequence Alignment; Signal Transduction; Vascular Endothelial Growth Factor Receptor-1 - antagonists & inhibitors; Vascular Endothelial Growth Factor Receptor-1 - genetics; Vascular Endothelial Growth Factor Receptor-1 - metabolism; 화학
• ISSN: 1976-6696; 1976-670X
• DOI: 10.5483/BMBRep.2017.50.4.165

Vascular endothelial growth factor (VEGF) is an essential cytokine that has functions in the formation of new blood vessels and regression of cardiac hypertrophy. VEGF/VEGF-receptor-1 (VEGFR1) signaling plays a key role in the regression of cardiac hypertrophy, whereas VEGF/VEGFR2 signaling leads to cardiac hypertrophy. In this study, we identified the prohypertrophic role of miR-374 using neonatal rat ventricular myocytes (NRVMs). Our results showed that overexpression of miR-374 activated G protein-coupled receptor-mediated prohypertrophic pathways by the inhibition of VEGFR1-dependent regression pathways. Luciferase assays revealed that miR-374 could directly target the 3'-untranslated regions of VEGFR1 and cGMP-dependent protein kinase-1. Collectively, these findings demonstrated that miR-374 was a novel pro-hypertrophic microRNA functioning to suppress the VEGFR1-mediated regression pathway. [BMB Reports 2017; 50(4): 208-213].