Isoenzyme analysis of Arthrobotrys, a nematode-trapping fungus

• Published in: Brazilian journal of medical and biological research Vol. 30; no. 10; pp. 1149 - 1152
• Main Authors: Araújo, J V, Junghans, T G, Alfenas, A C, Gomes, A P
• Format: Journal Article
• Language: English
• Published: Brazil Associação Brasileira de Divulgação Científica 01-10-1997
• Subjects: Animals; Arthrobotrys; BIOLOGY; electrophoresis; Fungi - enzymology; isoenzymes; Isoenzymes - analysis; MEDICINE, RESEARCH & EXPERIMENTAL; Nematoda - microbiology; nematode-trapping fungus; nematophagous fungi; predacious fungi; Arthrobotrys; nematode-trapping fungus; isoenzymes; electrophoresis; nematophagous fungi; predacious fungi
• ISSN: 0100-879X; 1414-431X; 0100-879X; 1414-431X
• DOI: 10.1590/S0100-879X1997001000001

Extraction and isoenzyme analysis of four isolates of Arthrobotrys including A. musiformis, A. robusta and A. conoides were conducted. Among the 14 enzymes studied by starch gel electrophoresis, using morpholine-citrate as gel/electrode buffer, the following nine enzymes showed interpretable banding patterns: alpha-esterase, fumarase, hexokinase, isocitrate dehydrogenase, leucine aminopeptidase, malate dehydrogenase, 6-phosphogluconate dehydrogenase, phosphoglucomutase and phosphoglucoisomerase. All isolates studied displayed typical isoenzyme phenotypes for each species. Two isolates of A. conoides differed in their alpha-isoesterase banding patterns, but no differences were observed for the other enzymes. The assay was satisfactory for enzyme extraction and resolution of Arthrobotrys and could be used in future taxonomic and genetic studies of this organism.