Combination of small molecule microarray and confocal microscopy techniques for live cell staining fluorescent dye discovery

• Published in: Molecules (Basel, Switzerland) Vol. 18; no. 8; pp. 9999 - 10013
• Main Authors: Molnár, Eszter, Kuntam, Soujanya, Cingaram, Pradeep Kumar Reddy, Peksel, Begüm, Suresh, Bhavyashree, Fábián, Gabriella, Fehér, Liliána Z, Bokros, Attila, Medgyesi, Agnes, Ayaydin, Ferhan, Puskás, László G
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 20-08-2013; MDPI
• Subjects: Biological research; confocal laser scanning microscopy; Dyes; fluorescent dye discovery; Fluorescent Dyes - chemistry; high-throughput screening; Lasers; Localization; Microscopy; Microscopy, Confocal - methods; Molecular Structure; Proteins; Scanners; small molecule microarray; Hungary
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules18089999

Discovering new fluorochromes is significantly advanced by high-throughput screening (HTS) methods. In the present study a combination of small molecule microarray (SMM) prescreening and confocal laser scanning microscopy (CLSM) was developed in order to discover novel cell staining fluorescent dyes. Compounds with high native fluorescence were selected from a 14,585-member library and further tested on living cells under the microscope. Eleven compartment-specific, cell-permeable (or plasma membrane-targeted) fluorochromes were identified. Their cytotoxicity was tested and found that between 1-10 micromolar range, they were non-toxic even during long-term incubations.