Dereplication of Components Coupled with HPLC-qTOF-MS in the Active Fraction of Humulus japonicus and It's Protective Effects against Parkinson's Disease Mouse Model

• Published in: Molecules (Basel, Switzerland) Vol. 24; no. 7; p. 1435
• Main Authors: Lee, Hee Ju, Dhodary, Basanta, Lee, Ju Yong, An, Jin-Pyo, Ryu, Young-Kyoung, Kim, Kyoung-Shim, Lee, Chul-Ho, Oh, Won Keun
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 11-04-2019; MDPI
• Subjects: Administration, Oral; Alzheimer's disease; Amine oxidase (flavin-containing); Animal behavior; Animals; Apoptosis; Chromatography; Chromatography, High Pressure Liquid; Colitis; Disease Models, Animal; Dysentery; Enzymatic activity; Enzyme activity; Enzymes; Ethanol; Flavones - administration & dosage; Flavones - chemistry; Flavonoids; Gene Expression Regulation - drug effects; Glucosides; Glucosides - administration & dosage; Glucosides - chemistry; High performance liquid chromatography; HPLC-qTOF-MS; Humans; Humulus - chemistry; Humulus japonicus; Hypertension; in vitro MAO B assay; in vivo 6-OHDA-lesioned PD animal test; Kinases; Liquid chromatography; Magnetic Resonance Spectroscopy; Mass spectroscopy; Mice; Molecular weight; Monoamine Oxidase - genetics; Motor Activity - drug effects; Motor Activity - genetics; NMR; Nuclear magnetic resonance; Oral administration; Oxidative stress; Oxidopamine - toxicity; Parkinson Disease, Secondary - chemically induced; Parkinson Disease, Secondary - drug therapy; Parkinson Disease, Secondary - genetics; Parkinson Disease, Secondary - pathology; Parkinson's disease; Plant Extracts - administration & dosage; Plant Extracts - chemistry; Retention; Tandem Mass Spectrometry; Tuberculosis; Waterborne diseases; Japan; HPLC-qTOF-MS; Parkinson’s Disease; Humulus japonicus; in vitro MAO B assay; in vivo 6-OHDA-lesioned PD animal test
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules24071435

is an annual plant belonging to the Cannabacea family, and it has been traditionally used to treat pulmonary tuberculosis, dysentery, chronic colitis, and hypertension. We investigated the active components against Parkinson's disease from
fraction (HJF) using high performance liquid chromatography (HPLC) coupled with quadruple-time-of-flight mass spectroscopy (qTOF-MS) and NMR. Fourteen compounds were isolated from HJF, including one new compound, using HPLC-qTOF-MS and NMR. The major compounds of HJF were luteolin-7-
-glucoside and apigenin-7-
-glucoside, and there was approximately 12.57- and 9.68-folds increase in the contents of these flavonoids compared to those of the 70% EtOH extract. Apigenin and luteolin exhibited the strongest inhibitory effects on monoamine oxidase (MAO) B enzyme activity. In animal studies, limb-use behavior was significantly reduced by unilateral 6-OHDA lesion and ipsilateral rotations. These results indicated that oral administration of 300 mg/kg HJF resulted in the improvement of motor asymmetry and motor impairment in unilateral 6-OHDA-lesioned mice. HJF, including active components leads to an improvement of motor behavior in a Parkinson's disease mouse model.