Dietary supplementation of old rats with hydrogenated peanut oil restores activities of mitochondrial respiratory complexes in skeletal muscles

• Published in: Biochemistry (Moscow) Vol. 75; no. 12; pp. 1491 - 1497
• Main Authors: Bronnikov, G. E, Kulagina, T. P, Aripovsky, A. V
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : SP MAIK Nauka/Interperiodica 01-12-2010; SP MAIK Nauka/Interperiodica; Springer; Springer Nature B.V
• Subjects: Age Factors; Animals; Arachis hypogaea; Biochemistry; Biomedical and Life Sciences; Biomedicine; Bioorganic Chemistry; Cytochrome c; Cytochrome oxidase; Diet; Dietary supplements; Electron Transport Chain Complex Proteins - metabolism; Enzyme Assays; Enzymes; Fatty acids; Fatty Acids - chemistry; Hydrogenation; Legumes; Life Sciences; Microbiology; Mitochondria; Mitochondria - drug effects; Mitochondria - enzymology; Muscle, Skeletal - chemistry; Muscle, Skeletal - drug effects; Muscle, Skeletal - enzymology; Muscles; Oils & fats; Oxidative stress; Peanut Oil; Plant Oils - administration & dosage; Plant Oils - pharmacology; Rats; Rats, Sprague-Dawley; Rodents; vegetable oil; mitochondrial respiratory chain; fatty acid composition of lipids; aging; oxidative stress; skeletal muscles
• ISSN: 0006-2979; 1608-3040
• DOI: 10.1134/S0006297910120102

The effect of dietary supplementation of old rats (26-33 months) with hydrogenated peanut oil on the activity of mitochondrial enzymes in skeletal muscles has been studied. The activities of NADH-coenzyme Q1 oxidoreductase, cytochrome c oxidase, and citrate synthase were determined spectrophotometrically in muscle homogenates. The activities of respiratory complexes I and IV were shown to significantly decrease with the age compared to the activity of the same enzymes in young animals, while the activity of citrate synthase was virtually unchanged. The fatty acid composition of muscle homogenates of old rats differed from that of young animals by a reduced content of myristic, oleic, linoleic, and α-linolenic acids and enhanced content of dihomo-γ-linolenic, arachidonic, and docosahexaenoic acids. Per oral supple-mentation of the old rats with hydrogenated peanut oil completely restored the activity of complex IV and increased the activity of complex I to 80% of the value observed in muscles of young animals, reducing the content of stearic, dihomo-γ-linolenic, arachidonic, eicosapentaenoic, docosapentaenoic, and docosahexaenoic acids relative to that in the groups of old and young rats. The content of oleic and linoleic acids increased relatively to that in the group of the old rats, as well as young animals. The possible mechanisms of the restoration of the activity of the respiratory enzymes under the administration of hydrogenated peanut oil are discussed.