Laboratory formulated magnetic nanoparticles for enhancement of viral gene expression in suspension cell line

• Published in: Journal of virological methods Vol. 147; no. 2; pp. 213 - 218
• Main Authors: Bhattarai, Shanta Raj, Kim, Sun Young, Jang, Kyu Yun, Lee, Ki Chang, Yi, Ho Keun, Lee, Dae Yeol, Kim, Hak Yong, Hwang, Pyoung Han
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 01-02-2008; Amsterdam Elsevier; New York, NY
• Subjects: Biological and medical sciences; Cell Line, Tumor; Chitosan; Ferric Compounds; Fundamental and applied biological sciences. Psychology; Gene; Gene Expression; Genes, Viral; Genetic Vectors; Humans; Iron oxide; Magnetic nanoparticles; Magnetics; Magnetofection; Microbiology; Nanoparticles; Techniques used in virology; Transfection - methods; Viral vectors; Virology; Viral vectors; Iron oxide; Chitosan; Gene; Magnetofection; Magnetic nanoparticles; Microbiology; Iron; Method; Gene expression; Virology; Cell line; Vector
• ISSN: 0166-0934; 1879-0984
• DOI: 10.1016/j.jviromet.2007.08.028

One factor critical to successful gene therapy is the development of efficient delivery systems. Although advances in gene transfer technology including viral and non-viral vectors have been made, an ideal vector system has not yet been constructed. Due to the growing concerns over the toxicity and immunogenicity of viral DNA delivery systems, DNA delivery via improve viral routes has become more desirable and advantageous. The ideal improve viral DNA delivery system should be a synthetic materials plus viral vectors. The materials should also be biocompatible, efficient, and modular so that it is tunable to various applications in both research and clinical settings. The successful steps towards this improve viral DNA delivery system is demonstrated: a magnetofection system mediated by modified cationic chitosan-coated iron oxide nanoparticles. Dense colloidal cationic iron oxide nanoparticles serve as an uptake-enhancing component by physical concentration at the cell surface in presence of external magnetic fields; enhanced viral gene expression (3–100-fold) due to the particles is seen as compared to virus vector alone with little virus dose.