Geographical Authentication of Macrohyporia cocos by a Data Fusion Method Combining Ultra-Fast Liquid Chromatography and Fourier Transform Infrared Spectroscopy

• Published in: Molecules (Basel, Switzerland) Vol. 24; no. 7; p. 1320
• Main Authors: Wang, Qin-Qin, Huang, Heng-Yu, Wang, Yuan-Zhong
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 03-04-2019; MDPI
• Subjects: Acids; authentication; Calibration; Chromatography; Chromatography, High Pressure Liquid; Chromatography, Liquid; Cocos - chemistry; Cocos - classification; data fusion; Data integration; Data Interpretation, Statistical; Discriminant analysis; Epidermis; Feasibility studies; Food quality; Fourier analysis; fourier transform infrared spectroscopy; Fourier transforms; Geographical distribution; Geography; Infrared spectra; Infrared spectroscopy; Liquid chromatography; Macrohyporia cocos; Metabolites; partial least squares discriminant analysis; Provenance; Quality control; Quantitative analysis; Spectroscopy, Fourier Transform Infrared; Spectrum analysis; Triterpenes - analysis; Triterpenes - chemistry; China; data fusion; liquid chromatography; Macrohyporia cocos; fourier transform infrared spectroscopy; partial least squares discriminant analysis; authentication
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules24071320

is a medicinal and edible fungi, which is consumed widely. The epidermis and inner part of its sclerotium are used separately.
quality is influenced by geographical origins, so an effective and accurate geographical authentication method is required. Liquid chromatograms at 242 nm and 210 nm (LC
and LC
) and Fourier transform infrared (FTIR) spectra of two parts were applied to authenticate the geographical origin of cultivated
combined with low and mid-level data fusion strategies, and partial least squares discriminant analysis. Data pretreatment involved correlation optimized warping and second derivative. The results showed that the potential of the chromatographic fingerprint was greater than that of five triterpene acids contents. LC
-FTIR low-level fusion took full advantage of information synergy and showed good performance. Further, the predictive ability of the FTIR low-level fusion model of two parts was satisfactory. The performance of the low-level fusion strategy preceded those of the single technique and mid-level fusion strategy. The inner parts were more suitable for origin identification than the epidermis. This study proved the feasibility of the data fusion of chromatograms and spectra, and the data fusion of different parts for the accurate authentication of geographical origin. This method is meaningful for the quality control of food and the protection of geographical indication products.