In-Vitro Cytotoxicity Study: Cell Viability and Cell Morphology of Carbon Nanofibrous Scaffold/Hydroxyapatite Nanocomposites

• Published in: Molecules (Basel, Switzerland) Vol. 26; no. 6; p. 1552
• Main Authors: Abd El-Aziz, Asmaa M, El-Maghraby, Azza, Ewald, Andrea, Kandil, Sherif H
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 11-03-2021; MDPI
• Subjects: Acids; Biocompatibility; Biomedical materials; Bone growth; Carbon; Carbon fibers; Carbonization; Cell adhesion & migration; cell counting; Cell culture; Cell growth; Cell morphology; Cell proliferation; Cell viability; Composite materials; Contact angle; Cytology; Cytotoxicity; Electrospinning; Fibroblasts; HA modifiedCNF membranes; Heat treatment; Heat treatments; Hydroxyapatite; Metabolism; Molecular weight; Morphology; Nanocomposites; Nanofibers; Polyacrylonitrile; Reagents; Regeneration; Regeneration (physiology); Scaffolds; Tissue engineering; Topography; WST test; St Louis Missouri; United States > US; Japan; Germany; WST test; HA modifiedCNF membranes; cell viability; cell counting; cell morphology; cytotoxicity
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules26061552

Electrospun carbon nanofibers (CNFs), which were modified with hydroxyapatite, were fabricated to be used as a substrate for bone cell proliferation. The CNFs were derived from electrospun polyacrylonitrile (PAN) nanofibers after two steps of heat treatment: stabilization and carbonization. Carbon nanofibrous (CNF)/hydroxyapatite (HA) nanocomposites were prepared by two different methods; one of them being modification during electrospinning (CNF-8HA) and the second method being hydrothermal modification after carbonization (CNF-8HA; hydrothermally) to be used as a platform for bone tissue engineering. The biological investigations were performed using in-vitro cell counting, WST cell viability and cell morphology after three and seven days. L929 mouse fibroblasts were found to be more viable on the hydrothermally-modified CNF scaffolds than on the unmodified CNF scaffolds. The biological characterizations of the synthesized CNF/HA nanofibrous composites indicated higher capability of bone regeneration.