Screening, Optimization, and Bioavailability Research of Natural Deep Eutectic Solvent Extracts from Radix Pueraria

• Published in: Molecules (Basel, Switzerland) Vol. 26; no. 3; p. 729
• Main Authors: Huang, Yan, Yang, Jiehong, Zhao, Yu, Yu, Li, He, Yu, Wan, Haitong, Li, Chang
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 31-01-2021; MDPI
• Subjects: Animals; Bioavailability; Biological Availability; Calibration; Drug Evaluation, Preclinical; Efficiency; green solvent; High-performance liquid chromatography; HPLC-MS; Hydrogen bonds; Ionic liquids; Isoflavones; Liquid chromatography; Malic acid; natural eutectic solvent; Natural products; Optimization; oral bioavailability; pharmacokinetic; Pharmacokinetics; Plant Extracts - chemistry; Plant Extracts - isolation & purification; Plant Extracts - pharmacokinetics; Plant Roots - chemistry; Plasma; Proline; Pueraria - chemistry; Radix Pueraria; Rats; Solubility; Solvent extraction; Solvents; Solvents - chemistry; Water - chemistry; puerarin; HPLC-MS; Radix Pueraria; natural eutectic solvent; extraction; green solvent; oral bioavailability; pharmacokinetic
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules26030729

Natural deep eutectic solvent (NaDES) is generally considered as a greener alternative to fossil solvent, with great potential in various areas. In the present work, 25 different NaDESs were screened for the extraction of puerarin (PUE) and its two natural derivatives from
(RP). As the main isoflavone in RP, PUE has a wide range of biological activities. However, its application is restricted due to its poor solubility in water and low oral bioavailability. In this study, the extraction of PUE with NaDESs showed significant advantages compared with traditional solvents. While using L-Pro-Maa (L-proline-malic acid) under optimal conditions, the optimized yields of PUE, 3-MPR and PRX were 98.7 mg/g, 16.3 mg/g and 9.9 mg/g, respectively, which were 2.2-, 2.9- and 3.4-fold higher than that of water. Furthermore, the oral bioavailability of PUE in NaDES extracts was comparatively investigated in rats with HPLC-MS technique. Pharmacokinetic analysis revealed that the relative bioavailability of PUE in L-Pro-Maa extract is 323%. The result indicated that NaDES is not only a sustainable ionic liquid with higher extraction efficiency, but also an enhancer of oral bioavailability of specific natural products.