Isolation and Identification of Bioactive Compounds from Bidens spp. Using HPLC-DAD and GC-MS Analysis and Their Biological Activity as Anticancer Molecules

• Published in: Molecules (Basel, Switzerland) Vol. 27; no. 6; p. 1927
• Main Authors: Zahara, Kulsoom, Bibi, Yamin, Masood, Saadia, Nisa, Sobia, Sher, Ahmad, Ali, Naushad, Kumar, Sunjeet, Qayyum, Abdul, Ahmed, Waseem, Sami, Rokayya, Al-Mushhin, Amina A M, Aljahani, Amani H
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 16-03-2022; MDPI
• Subjects: Adenocarcinoma; Alveoli; anticancer; Antitumor activity; Artemia; Asthma; Bidens; Bioactive compounds; Biological activity; brine shrimp; Cancer; Cervical cancer; Cervix; Chemotherapy; Chromatography, High Pressure Liquid; Cytotoxicity; Epithelial cells; Epithelium; Ethanol; Eye diseases; Flavonoids; Gas Chromatography-Mass Spectrometry; HeLa Cells; Hepatocellular carcinoma; High-performance liquid chromatography; Humans; Liquid chromatography; Metabolism; Oxidative stress; Plant Extracts; Plants; Signal transduction; Tropical diseases; zebrafish; Cuba; Central America; Cameroon; St Louis Missouri; United States > US; China; Africa; Hong Kong China; Uganda; Germany; India; Bidens; zebrafish; brine shrimp; anticancer
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules27061927

The genus
a member of family Compositae, is widely documented as an ethno-medicinally important genus of plants. In the present study, anticancer potential of three ethno-medicinally important species i.e.,
,
and
were tested. For in-vitro evaluation, an MTT (Thiazolyl blue tetrazolium bromide) assay was performed against cervical cancer cells (HeLa), hepatocellular carcinoma (HepG), and adenocarcinoma human alveolar basal epithelial cells (A549). For in vivo evaluation,
,
and
were used. Among all the tested extracts, the ethanol extract of
appeared to have highest anticancer activity, and the compounds responsible for this activity were identified to be Tris (2,4-di-
-butylphenyl), 4-hydroxy-2,4'-dimethoxychalcone, and 2,4-di-
-butylphenol. This is the first report of the isolation of Tris (2,4-di-
-butylphenyl) phosphate from the genus
and the first report of 4-hydroxy-2,4'-dimethoxychalcone and 2,4-di-
-butylphenol from
. Among the isolated compounds, 4-hydroxy-2,4'-dimethoxychalcone showed the highest anticancer activity with an LD50 value of 236.7 µg/mL. Therefore, this compound carries promising potential for being established as a pharmaceutical for chemoprevention and chemotherapy.