An Effective Chromatography Process for Simultaneous Purification and Separation of Total Lignans and Flavonoids from Valeriana amurensis

• Published in: Molecules (Basel, Switzerland) Vol. 27; no. 23; p. 8598
• Main Authors: Zhang, Manli, Yang, Bingyou, Ye, Minhui, Chen, Jianqing, Liu, Yan, Wang, Changfu
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 06-12-2022; MDPI
• Subjects: Adsorption; Chromatography; Chromatography, High Pressure Liquid; Column chromatography; Ethanol; Flavonoids; Flavonoids - chemistry; Flow velocity; High performance liquid chromatography; Insomnia; Lignans; Liquid chromatography; macroporous resin; Nervous system; Neurological disorders; Plant Extracts - chemistry; polyamide resin; Polyamide resins; Polyamides; Purification; Resins; Resins, Plant - chemistry; Resins, Synthetic - chemistry; Separation; Valeriana amurensis; Water; macroporous resin; flavonoids; Valeriana amurensis; polyamide resin; lignans
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules27238598

An effective chromatography process was developed and validated for simultaneous purification and separation of total lignans and flavonoids from
. The total lignans and flavonoids in
extract were prepurified with macroporous resin column chromatography, and the conditions were optimized as follows: 40 mg/mL
extract (2.0 g) solution was loaded onto an AB-8 resin column with a diameter-to-height ratio of 1:7, followed by adsorption for 6 h; then, the column was eluted successively with 5 BV water and 10% and 50% ethanol at a flow rate 2 BV/h. The obtained 50% ethanol fraction was further repurified and separated by polyamide resin column chromatography to obtain the total lignans and flavonoids, respectively. The chromatography conditions were optimized as follows: a 50% ethanol fraction (1.0 g) was mixed with 1.0 g polyamide resin and loaded onto a polyamide resin (60-100 mesh) column with a diameter-to-height ratio of 1:3; then, the column was eluted successively with 6 BV water and 40% and 80% ethanol at a flow rate of 4 BV/h. The total lignans and flavonoids were obtained from water and 80% ethanol fraction, respectively. The content and recovery of standard compounds in total lignans and flavonoids were analyzed with HPLC-PDA, and the feasibility of the process was confirmed.