Primary structure of bovine conglutinin, a member of the C-type animal lectin family

• Published in: The Journal of biological chemistry Vol. 266; no. 5; pp. 2715 - 2723
• Main Authors: Lee, Y.M. (Applied Immune Sciences, Menlo Park, CA), Leiby, K.R, Allar, J, Paris, K, Lerch, B, Okarma, T.B
• Format: Journal Article
• Language: English
• Published: Bethesda, MD American Society for Biochemistry and Molecular Biology 15-02-1991
• Subjects: Amino Acid Sequence; Analytical, structural and metabolic biochemistry; Animals; Binding and carrier proteins; Biological and medical sciences; BOVIDAE; Carbohydrate Metabolism; Cattle; Collectins; Fundamental and applied biological sciences. Psychology; Glycosylation; LECTINA; LECTINE; Lectins - genetics; Molecular Sequence Data; Proteins; Sequence Alignment; Sequence Homology, Nucleic Acid; Serum Globulins - genetics; Serum Globulins - metabolism; Lectin; Binding protein; Vertebrata; Mammalia; Bovine; Molecular structure; Collagen; Edman degradation; HPLC chromatography; Artiodactyla; Ungulata; Aminoacid sequence
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1016/s0021-9258(18)49904-8

We report the complete amino acid sequence of bovine conglutinin obtained by structural characterization of peptides derived from the protein by various chemical and enzymatic fragmentation methods. The protein consists of 351 amino acid residues including 55 apparent Gly-X-Y repeats with two interruptions. This 171-residue-long collagenous domain separates a short noncollagenous NH2-terminal region of 25 residues from the 155-residue-long globular COOH terminus revealing the structural relation of conglutinin with mannose-binding proteins, pulmonary surfactant-associated proteins, and a complement component C1q. Eight hydroxylysine residues were found in the collagenous domain. All of these hydroxylysine residues which occupy a Y position in a Gly-X-Y triplet are possible glycosylation sites since no phenylthiohydantoin amino acid was identified in automated Edman degradation cycles corresponding to these sites. The noncollagenous COOH domain of conglutinin, on the other hand, contains a carbohydrate recognition domain which shares substantial sequence homology with C-type animal lectins. Conglutinin has the greatest sequence similarity with mannose-binding proteins and pulmonary surfactant-associated proteins