Disparate macrophage responses are linked to infection outcome of Hantan virus in humans or rodents

• Published in: Nature communications Vol. 15; no. 1; p. 438
• Main Authors: Ma, Hongwei, Yang, Yongheng, Nie, Tiejian, Yan, Rong, Si, Yue, Wei, Jing, Li, Mengyun, Liu, He, Ye, Wei, Zhang, Hui, Cheng, Linfeng, Zhang, Liang, Lv, Xin, Luo, Limin, Xu, Zhikai, Zhang, Xijing, Lei, Yingfeng, Zhang, Fanglin
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 10-01-2024; Nature Publishing Group; Nature Portfolio
• Subjects: 13/1; 13/31; 13/89; 13/95; 14/19; 14/32; 38/91; 631/250/2504/342; 631/326/596/2553; 631/326/596/2555; 64/110; 64/60; 692/699/255/2514; Ablation; Asymptomatic infection; Cytokine storm; Disease transmission; Fever; Hemorrhagic fever with renal syndrome; Humanities and Social Sciences; Inactivation; Infections; Infectious diseases; Inflammation; Macrophages; multidisciplinary; Myeloid cells; Negative feedback; NF-κB protein; Non-coding RNA; Notch protein; Phenotypes; Phosphorylation; Receptors; Rodents; Science; Science (multidisciplinary); Viruses
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-024-44687-4

Hantaan virus (HTNV) is asymptomatically carried by rodents, yet causes lethal hemorrhagic fever with renal syndrome in humans, the underlying mechanisms of which remain to be elucidated. Here, we show that differential macrophage responses may determine disparate infection outcomes. In mice, late-phase inactivation of inflammatory macrophage prevents cytokine storm syndrome that usually occurs in HTNV-infected patients. This is attained by elaborate crosstalk between Notch and NF-κB pathways. Mechanistically, Notch receptors activated by HTNV enhance NF-κB signaling by recruiting IKKβ and p65, promoting inflammatory macrophage polarization in both species. However, in mice rather than humans, Notch-mediated inflammation is timely restrained by a series of murine-specific long noncoding RNAs transcribed by the Notch pathway in a negative feedback manner. Among them, the lnc-ip65 detaches p65 from the Notch receptor and inhibits p65 phosphorylation, rewiring macrophages from the pro-inflammation to the pro-resolution phenotype. Genetic ablation of lnc-ip65 leads to destructive HTNV infection in mice. Thus, our findings reveal an immune-braking function of murine noncoding RNAs, offering a special therapeutic strategy for HTNV infection.
Hantaan virus is carried and transmitted by rodents and results in asymptomatic infection, yet transmission to humans’ results in symptomatic disease and development of hemorrhagic fever with renal syndrome. Here the authors explore the disparate effects in myeloid cells from mice and humans.