In vitro and in vivo antioxidant activity of a water-soluble polysaccharide from Dendrobium denneanum

• Published in: Molecules (Basel, Switzerland) Vol. 16; no. 2; pp. 1579 - 1592
• Main Authors: Luo, Aoxue, Ge, Zhongfu, Fan, Yijun, Luo, Aoshuang, Chun, Ze, He, Xingjin
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 14-02-2011; MDPI
• Subjects: Animals; antioxidant activity; Antioxidants; Antioxidants - chemistry; Antioxidants - metabolism; Benzothiazoles - chemistry; Benzothiazoles - metabolism; Dendrobium - anatomy & histology; Dendrobium - chemistry; Dendrobium denneanum; Glucose; Hydroxyl Radical - chemistry; Hydroxyl Radical - metabolism; Mice; Molecular weight; Monosaccharides - analysis; Nuclear Magnetic Resonance, Biomolecular; Plant Extracts - chemistry; Plant Extracts - metabolism; Plant Stems - chemistry; polysaccharide; Polysaccharides - chemistry; Polysaccharides - metabolism; Random Allocation; Spectroscopy, Fourier Transform Infrared; Sulfonic Acids - chemistry; Sulfonic Acids - metabolism; Superoxide Dismutase - metabolism; Water - chemistry; United States > US; China
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules16021579

The water-soluble crude polysaccharide (DDP) obtained from the aqueous extracts of the stem of Dendrobium denneanum through hot water extraction followed by ethanol precipitation, was found to have an average molecular weight (Mw) of about 484.7 kDa. Monosaccharide analysis revealed that DDP was composed of arabinose, xylose, mannose, glucose and galactose in a molar ratio of 1.00:2.66:8.92:34.20:10.16. The investigation of antioxidant activity both in vitro and in vivo showed that DDP is a potential antioxidant.