Heliorhodopsin-mediated light-modulation of ABC transporter

• Published in: Nature communications Vol. 15; no. 1; p. 4306
• Main Authors: Cho, Shin-Gyu, Kim, Ji-Hyun, Lee, Ji-eun, Choi, In-Jung, Song, Myungchul, Chuon, Kimleng, Shim, Jin-gon, Kang, Kun-Wook, Jung, Kwang-Hwan
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 21-05-2024; Nature Publishing Group; Nature Portfolio
• Subjects: 45/43; 631/45/612/1237; 631/535/1267; 631/57/2272/951; 82/16; 82/29; 82/51; 82/80; 82/81; 82/83; ABC transporter; ATP-Binding Cassette Transporters - genetics; ATP-Binding Cassette Transporters - metabolism; ATP-binding protein; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Binding; Coding; Drug resistance; Genes; Humanities and Social Sciences; Light; Light effects; Modulation; multidisciplinary; Multidrug resistance; Optogenetics - methods; Protein Binding; Protein interaction; Proteins; Residues; Rhodopsin; Rhodopsins, Microbial - chemistry; Rhodopsins, Microbial - genetics; Rhodopsins, Microbial - metabolism; Science; Science (multidisciplinary)
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-024-48650-1

Heliorhodopsins (HeRs) have been hypothesized to have widespread functions. Recently, the functions for few HeRs have been revealed; however, the hypothetical functions remain largely unknown. Herein, we investigate light-modulation of heterodimeric multidrug resistance ATP-binding cassette transporters (OmrDE) mediated by
Omithinimicrobium cerasi
HeR. In this study, we classifiy genes flanking the HeR-encoding genes and identify highly conservative residues for protein–protein interactions. Our results reveal that the interaction between OcHeR and OmrDE shows positive cooperatively sequential binding through thermodynamic parameters. Moreover, light-induced OcHeR upregulates OmrDE drug transportation. Hence, the binding may be crucial to drug resistance in
O. cerasi
as it survives in a drug-containing habitat. Overall, we unveil a function of HeR as regulatory rhodopsin for multidrug resistance. Our findings suggest potential applications in optogenetic technology.
Here, authors classify genes flanking the Heliorhodopsin (HeR)-encoding genes and identify highly conserved residues for protein–protein interactions, revealing a function of HeR as regulatory rhodopsin for multidrug resistance.