Serum anti-PCK1 antibody levels are a prognostic factor for patients with diabetes mellitus

• Published in: BMC endocrine disorders Vol. 23; no. 1; pp. 1 - 239
• Main Authors: Namiki, Toshiki, Takemoto, Minoru, Hayashi, Aiko, Yamagata, Hiroki, Ishikawa, Takahiro, Yokote, Koutaro, Li, Shu-Yang, Kubota, Masaaki, Zhang, Bo-Shi, Yoshida, Yoichi, Matsutani, Tomoo, Mine, Seiichiro, Machida, Toshio, Kobayashi, Yoshio, Terada, Jiro, Naito, Akira, Tatsumi, Koichiro, Takizawa, Hirotaka, Nakamura, Rika, Kuroda, Hideyuki, Iwadate, Yasuo, Hiwasa, Takaki
• Format: Journal Article
• Language: English
• Published: London BioMed Central Ltd 30-10-2023; BioMed Central; BMC
• Subjects: Angina pectoris; Antibodies; Antibody biomarker; Antigens; Apnea; Arteriosclerosis; Atherosclerosis; Autoantibodies; Autoimmune diseases; Autoimmunity; Blood pressure; Cardiovascular disease; Cardiovascular diseases; Care and treatment; Chromatography; Development and progression; Diabetes; Diabetes mellitus; Diabetics; Disease; Heart attacks; Hospitals; Immunoglobulin G; Ischemia; Medical research; Medicine, Experimental; Patients; Phosphoenolpyruvate carboxykinase 1; Prognosis; Proteins; Serology; Sleep apnea syndromes; Sleep disorders; Stroke; Stroke (Disease); Survival; Transient ischemic attack; Taiwan; La Jolla California; United States > US; Japan
• ISSN: 1472-6823; 1472-6823
• DOI: 10.1186/s12902-023-01491-3

Background Autoantibodies develop in autoimmune diseases, cancer, diabetes mellitus (DM), and atherosclerosis-related diseases. However, autoantibody biomarkers have not been successfully examined for diagnosis and therapy. Methods Serological identification of antigens through recombinant cDNA expression cloning (SEREX) was used for primary screening of antigens. The cDNA product was expressed in bacteria and purified. Amplified luminescent proximity homogeneous assay-linked immunosorbent assay (AlphaLISA) was used to evaluate antibody levels in serum samples. Results Phosphoenolpyruvate carboxykinase 1 (PCK1) was recognized as an antigen by serum IgG antibodies in the sera of patients with atherosclerosis. AlphaLISA showed significantly higher serum antibody levels against recombinant PCK1 protein in patients with DM and cardiovascular disease than in healthy donors, but not in those with acute ischemic stroke, transient ischemic attack, or obstructive sleep apnea syndrome. The area under the receiver operating characteristic curve for anti-PCK1 antibodies was 0.7024 for DM. The serum anti-PCK1 antibody levels were associated with age, platelet count, and blood pressure. Anti-PCK1-antibody-positive patients showed significantly lower overall survival than the negative patients. Conclusions Serum anti-PCK1 antibody levels were found to be associated with DM. The anti-PCK1 antibody marker is useful for predicting the overall survival of patients with DM. Keywords: Phosphoenolpyruvate carboxykinase 1, Diabetes mellitus, Atherosclerosis, Cardiovascular disease, Antibody biomarker