Oxygen consumption rate v. rate of energy utilization of fishes: a comparison and brief history of the two measurements

• Published in: Journal of fish biology Vol. 88; no. 1; pp. 10 - 25
• Main Author: Nelson, J. A.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01-01-2016; Wiley Subscription Services, Inc
• Subjects: Animals; calorimetry; Calorimetry, Indirect - history; Carbon Dioxide - metabolism; Consumption; Energy Metabolism; fish; Fishes - physiology; history; History, 18th Century; History, 19th Century; History, 20th Century; metabolic rate; Metabolism; Oxygen; Oxygen - metabolism; Oxygen Consumption; metabolic rate; calorimetry; metabolism; history; fish
• ISSN: 0022-1112; 1095-8649
• DOI: 10.1111/jfb.12824

Accounting for energy use by fishes has been taking place for over 200 years. The original, and continuing gold standard for measuring energy use in terrestrial animals, is to account for the waste heat produced by all reactions of metabolism, a process referred to as direct calorimetry. Direct calorimetry is not easy or convenient in terrestrial animals and is extremely difficult in aquatic animals. Thus, the original and most subsequent measurements of metabolic activity in fishes have been measured via indirect calorimetry. Indirect calorimetry takes advantage of the fact that oxygen is consumed and carbon dioxide is produced during the catabolic conversion of foodstuffs or energy reserves to useful ATP energy. As measuring [CO2] in water is more challenging than measuring [O2], most indirect calorimetric studies on fishes have used the rate of O2 consumption. To relate measurements of O2 consumption back to actual energy usage requires knowledge of the substrate being oxidized. Many contemporary studies of O2 consumption by fishes do not attempt to relate this measurement back to actual energy usage. Thus, the rate of oxygen consumption (M˙O2) has become a measurement in its own right that is not necessarily synonymous with metabolic rate. Because all extant fishes are obligate aerobes (many fishes engage in substantial net anaerobiosis, but all require oxygen to complete their life cycle), this discrepancy does not appear to be of great concern to the fish biology community, and reports of fish oxygen consumption, without being related to energy, have proliferated. Unfortunately, under some circumstances, these measures can be quite different from one another. A review of the methodological history of the two measurements and a look towards the future are included.