Chromosomal assignment and expression pattern of the murine Lasp-1 gene

• Published in: Gene Vol. 207; no. 2; pp. 171 - 175
• Main Authors: Schreiber, Valérie, Masson, Régis, Linares, José Luis, Mattei, Marie-Geneviève, Tomasetto, Catherine, Rio, Marie-Christine
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 30-01-1998; Elsevier
• Subjects: Adaptor Proteins, Signal Transducing; Aging - metabolism; Amino Acid Sequence; Animals; Biochemistry, Molecular Biology; cDNA cloning; Cells, Cultured; Chromosome in situ hybridization; Chromosome Mapping; Cytoskeletal Proteins; DNA, Complementary; Embryonic and Fetal Development; Gene Expression; Homeodomain Proteins - genetics; Humans; Life Sciences; LIM domain; LIM Domain Proteins; Male; Mice; Molecular Sequence Data; Neoplasm Proteins; Organ Specificity; Restriction Mapping; SH3 domain; src Homology Domains; SH3 domain; Lasp-1, LIM and SH3 protein-1; LIM, Lin-11, Isl-1, Mec-3; ES cells, embryonic stem cells; kb, kilobase(s) or 1000 bp; bp, base pair(s); SH3, Src homology 3; Gene expression; cDNA cloning; LIM domain; SH2, Src homology 2; Chromosome in situ hybridization; CRP, cysteine-rich protein; dpc, days post-coitum; ARGBP2, Arg/Abl-binding protein 2; BRCA1, breast cancer susceptibility gene 1; CRIP, cysteine-rich intestinal protein; c-erbB-2, oncogene of the epidermal growth factor receptor family
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/S0378-1119(97)00622-7

The human
Lasp-1 (
LIM
and
SH3
protein) gene was previously identified by differential screening of a breast cancer-derived metastatic lymph node cDNA library. It was located on the q12–q21 region of human chromosome 17 and was shown to be amplified and overexpressed in 12% of breast tumours. Lasp-1 defines a new LIM-protein subfamily, as it associates a C-terminal
Src homology 3 (SH3) domain to a N-terminal LIM motif. In this study, the isolation and characterization of the cDNA encoding the mouse Lasp-1 protein are described, and it is shown to be highly conserved with its human counterpart. In addition to the LIM and SH3 domains, both human and mouse Lasp-1 contain an actin-binding domain. The mouse gene was mapped by in situ hybridization to the 11C–11D region of chromosome 11. Northern blot analysis shows that this gene is expressed from 7.5 to 17.5
days post-coitum of mouse embryogenesis and in almost all adult tissues.