Assessment of mitochondria as a compartment for phosphatidylinositol synthesis in Solanum tuberosum

• Published in: Plant physiology and biochemistry Vol. 48; no. 12; pp. 952 - 960
• Main Authors: Davy de Virville, Jacques, Brown, Spencer, Cochet, Françoise, Soler, Marie-Noëlle, Hoffelt, Michèle, Ruelland, Eric, Zachowski, Alain, Collin, Sylvie
• Format: Journal Article
• Language: English
• Published: Paris Elsevier Masson SAS 01-12-2010; Elsevier
• Subjects: Biological and medical sciences; CDP-Diacylglycerol-Inositol 3-Phosphatidyltransferase; CDP-Diacylglycerol-Inositol 3-Phosphatidyltransferase - metabolism; Endoplasmic Reticulum; Endoplasmic Reticulum - metabolism; Fundamental and applied biological sciences. Psychology; GFP; Life Sciences; Mitochondria; Mitochondria - enzymology; Mitochondria - metabolism; Mitochondrial Membranes; Mitochondrial Membranes - metabolism; Outer mitochondrial membrane; Phosphatidylinositol synthase; Phosphatidylinositols; Phosphatidylinositols - biosynthesis; Phospholipid synthesis; Plant physiology and development; Solanum tuberosum; Solanum tuberosum - metabolism; Sub-cellular localization; Vegetal Biology; ST; YFP; IM; Solanum tuberosum; Outer mitochondrial membrane; CP; ER; Phospholipid synthesis; GFP; Mitochondria; CCCP; CDP-alcohol; Phosphatidylinositol synthase; MAM; DiOC; Sub-cellular localization; GA; PtdIns; FA; PIS; PM; OM; PtdCho; Evaluation; Enzyme; Phospholipid; Outer; Synthase; Phosphatidylinositol; Plant physiology; Dicotyledones; Angiospermae; Membrane; Spermatophyta; Tuber plant; Solanaceae; Localization
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2010.09.004

The outer mitochondrial membrane is particularly rich in phosphatidylinositol (PtdIns), a phospholipid found in different amounts in all eukaryotic membranes, but not synthesized in situ by all. PtdIns is therefore subjected to traffic from the synthesizing membranes to the non-synthesizing ones. The contribution of mitochondria to the cell PtdIns pool has never been the focus of a specific study in plants, whereas in yeast, the presence of the enzyme responsible for synthesis, PtdIns synthase (PIS, cytidine 5′-diphospho-1,2-diacyl-
sn-glycerol:
myo-inositol 3-phosphatidyltransferase, EC 2.7.8.11), has clearly been demonstrated in mitochondria. As these organelles have now been shown to be responsible for the synthesis of several lipids, the present work aimed at evaluating mitochondria as a compartment for the synthesis of PtdIns in plants. The sub-cellular localization of PIS was studied in
Solanum tuberosum L. by membrane fractionation, enzymatic analysis and by confocal microscopy in living cells. In potato, beside the endoplasmic reticulum, the activity of PIS was found to be tightly associated to mitochondria. Using a fluorescent reporter fusion, the enzyme was also found to be associated to these organelles. The enzyme was not present at the plasma membrane. A comparison of the localization in other cell systems suggests that the mitochondrial localization could be regulated.
► Phosphatidylinositol synthase is associated to mitochondria in potato leaf and tubers. ► Association of PIS to mitochondria depends on the cell type. ► StPIS1 is identified as a functional PIS.