O-GlcNAc transferase (OGT) as a placental biomarker of maternal stress and reprogramming of CNS gene transcription in development

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 110; no. 13; pp. 5169 - 5174
• Main Authors: Howerton, Christopher L., Morgan, Christopher P., Fischer, David B., Bale, Tracy L.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 26-03-2013; National Acad Sciences
• Subjects: Animals; Biological markers; Biological Sciences; Biomarkers; Biomarkers - metabolism; Chromatin; Chromosomes, Human, X - genetics; Chromosomes, Human, X - metabolism; Confidence interval; Datasets; Embryo, Mammalian - embryology; Embryo, Mammalian - enzymology; Embryo, Mammalian - pathology; Epigenesis, Genetic - genetics; Female; Gene expression; Gene expression regulation; Gene Expression Regulation, Developmental; Genes; Humans; Hypothalamus - embryology; Hypothalamus - enzymology; Hypothalamus - pathology; Male; Mice; Mice, Transgenic; MicroRNA; N-Acetylglucosaminyltransferases - biosynthesis; Neurogenesis - genetics; Neurological disorders; Placenta; Placenta - enzymology; Placenta - pathology; Pregnancy; Pregnancy Complications - enzymology; Pregnancy Complications - genetics; Pregnancy Complications - pathology; Pregnancy Proteins - biosynthesis; Pregnancy Proteins - genetics; Proteins; Risk factors; Rodents; Sex Characteristics; Sex linked differences; Stress, Physiological
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.1300065110

Maternal stress is a key risk factor for neurodevelopmental disorders, including schizophrenia and autism, which often exhibit a sex bias in rates of presentation, age of onset, and symptom severity. The placenta is an endocrine tissue that functions as an important mediator in responding to perturbations in the intrauterine environment and is accessible for diagnostic purposes, potentially providing biomarkers predictive of disease. Therefore, we have used a genome-wide array approach to screen placental expression across pregnancy for gene candidates that are sex-biased and stress-responsive in mice and translate to human tissue. We identifed O-linked- N -acetylglucosamine (O-GlcNAc) transferase (OGT), an X-linked gene important in regulating proteins involved in chromatin remodeling, as fitting these criteria. Levels of both OGT and its biochemical mark, O - GlcNAcylation, were significantly lower in males and further reduced by prenatal stress. Examination of human placental tissue found similar patterns related to X chromosome dosage. As a demonstration of the importance of placental OGT in neurodevelopment, we found that hypothalamic gene expression and the broad epigenetic microRNA environment in the neonatal brain of placental-specific hemizygous OGT mice was substantially altered. These studies identified OGT as a promising placental biomarker of maternal stress exposure that may relate to sex-biased outcomes in neurodevelopment.