Effects of Bacillus subtilis CSL2 on the composition and functional diversity of the faecal microbiota of broiler chickens challenged with Salmonella Gallinarum

• Published in: Journal of animal science and biotechnology Vol. 8; no. 2; pp. 450 - 458
• Main Authors: Oh, Ju Kyoung, Pajarillo, Edward Alain B, Chae, Jong Pyo, Kim, In Ho, Yang, Dong Soo, Kang, Dae-Kyung
• Format: Journal Article
• Language: English
• Published: England BioMed Central 05-01-2017; Department of Animal Resources Science, Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of Korea%Abson BioChem, Inc, 10-1 Yangjimaeul-gil, Sangrok-gu, Ansan 15524, Republic of Korea
• Subjects: Animals; Bacillus; Diet; Discriminant analysis; Feeds; Homeostasis; Infections; Microbiota; Microorganisms; Pathogens; Phylogenetics; Poultry; Probiotics; Salmonella; Software; Taxonomy; Titanium; 伤寒沙门氏菌; 功能多样性; 微生物生态学; 枯草芽孢杆菌; 粪便菌群; 组成; 肉鸡日粮; Broiler chicken; Bacillus subtilis; Salmonella Gallinarum; 16S rRNA gene; Microbiome
• ISSN: 1674-9782; 2049-1891; 2049-1891
• DOI: 10.1186/s40104-016-0130-8

Background： The chicken gastrointestinal tract contains a diverse microbiota whose composition and structure play important roles in gut functionality. In this study, microbial shifts resulting from feed supplementation with Bacillus subtilis CSL2 were evaluated in broilers challenged and unchallenged with Salmonella Gallinarum. To analyse bacterial community composition and functionality, 454 GS-FLX pyrosequencing of 16 S r RNA gene amplicons was performed.Results： The Quantitative Insights into Microbial Ecology（QIIME） pipeline was used to analyse changes in the faecal microbiota over a 24-h period. A total of 718,204 sequences from broiler chickens were recorded and analysed. At the phylum level, Firmicutes, Bacteroidetes, and Proteobacteria were the predominant bacterial taxa. In Salmonellainfected chickens（SC）, Bacteroidetes were more highly abundant compared to control（NC） and Bacillus-treated（BT）chickens. At the genus level, in the NC and BT groups, Lactobacil us was present at high abundance, and the abundance of Turicibacter, unclassified Enterobacteriaceae, and Bacteroides increased in SC broilers. Furthermore, taxon-independent analysis showed that the SC and BT groups were compositional y distinct at the end of the 24-h period. Further analysis of functional properties showed that B. subtilis CSL2 administration increased gut-associated energy supply mechanisms（i.e. carbohydrate transport and metabolism） to maintain a stable microbiota and protect gut integrity.Conclusions： This study demonstrated that S. Gallinarum infection and B. subtilis CSL2 supplementation in the diet of broiler chickens influenced the diversity, composition, and functional diversity of the faecal microbiota. Moreover, the findings offer significant insights to understand potential mechanisms of Salmonel a infection and the mode of action of probiotics in broiler chickens.