In Vitro Antimicrobial and Antiproliferative Activity of Amphipterygium adstringens

• Published in: Evidence-based complementary and alternative medicine Vol. 2015; no. 2015; pp. 1 - 7
• Main Authors: Aviles-Arnaut, H., De la Torre-Zavala, S., Verde-Star, M. J., Teixeira, I. P., Rodriguez-Garcia, A., Ruiz, A. L. T. G.
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01-01-2015; John Wiley & Sons, Inc; Hindawi Limited
• Subjects: Antimicrobial agents; Biofilms; Cancer therapies; Candida albicans; Candida dubliniensis; Care and treatment; Chronic diseases; Chronic illnesses; Colleges & universities; Dental caries; Folk medicine; Gastritis; Health aspects; HIV; Human immunodeficiency virus; Infections; Medicine, Primitive; Microorganisms; Oral diseases; Porphyromonas gingivalis; Streptococcus mutans; Mexico
• ISSN: 1741-427X; 1741-4288
• DOI: 10.1155/2015/175497

Amphipterygium adstringens is a plant widely used in Mexican traditional medicine for its known anti-inflammatory and antiulcer properties. In this work, we evaluated the in vitro antimicrobial and antiproliferative activities of the methanolic extract of A. adstringens against oral pathogens such as Streptococcus mutans, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Candida albicans, and Candida dubliniensis, using microdilution (MIC) and agar diffusion methods (MBC), and the antiproliferative activity evaluating total growth inhibition (TGI) by staining the protein content with sulforhodamine B (SRB), using nine human cancer cell lines. Crude extract (CE) of A. adstringens showed some degree of activity against one or more of the strains with a MIC from 0.125 mg/mL to 63 mg/mL and MBC from 1.6 to 6.3 mg/mL and cytotoxic activity, particularly against NCI-ADR/RES, an ovarian cell line expressing multiple resistance drugs phenotype. The CE is a complex mixture of possible multitarget metabolites that could be responsible for both antimicrobial and antiproliferative activities, and further investigation is required to elucidate the identity of active compounds. Nevertheless the CE itself is useful in the development of new antimicrobial treatment based on natural products to prevent oral diseases and as alternative natural source for cancer treatment and prevention.