Evaluation of two commercial assays for the detection of Chlamydophila abortus antibodies

• Published in: Veterinary microbiology Vol. 123; no. 1; pp. 153 - 161
• Main Authors: Vretou, E., Radouani, F., Psarrou, E., Kritikos, I., Xylouri, E., Mangana, O.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 20-07-2007; Elsevier Science
• Subjects: abortion (animals); Abortion, Veterinary - diagnosis; Abortion, Veterinary - microbiology; Animals; antibodies; Antibodies, Bacterial - immunology; Antibodies, Bacterial - isolation & purification; antibody detection; assays; bacterial infections; Bacteriology; Biological and medical sciences; Chlamydophila - immunology; Chlamydophila - isolation & purification; Chlamydophila abortus; Chlamydophila Infections - diagnosis; Chlamydophila Infections - immunology; Chlamydophila Infections - veterinary; Chlamydophila pecorum; Chlamydophila psittaci; complement fixation tests; Complement Fixation Tests - veterinary; diagnostic techniques; enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - veterinary; ewes; Female; Fundamental and applied biological sciences. Psychology; gravid females; Indirect ELISA; lambs; Microbiology; Miscellaneous; Ovine enzootic abortion; Placenta - microbiology; product evaluation; recombinant proteins; Sensitivity and Specificity; serodiagnosis; Serologic Tests - veterinary; Serological diagnosis; Sheep; sheep diseases; Sheep Diseases - microbiology; Ovine enzootic abortion; Chlamydophila abortus; Serological diagnosis; Indirect ELISA; Chlamydophila pecorum; Farming animal; Chlamydiaceae; Pregnancy disorders; Microbiology; Antibody; Abortion; Vertebrata; Mammalia; Chlamydiales; Bacteria; Sheep; Artiodactyla; Diagnosis; Detection; Veterinary; ELISA assay; Ungulata
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/j.vetmic.2007.02.023

Two commercial enzyme-linked immunosorbent assays (ELISA), the CHEKIT
®-CHLAMYDIA which uses inactivated
Chlamydophila psittaci antigen, and the
Chlamydophila abortus ELISA produced by the Institut Pourquier which uses a recombinant fragment of the 80–90
kDa protein, were evaluated with the objective to determine whether the new ELISAs would perform as improved alternatives to the complement fixation test (CFT) for the serological diagnosis of ovine enzootic abortion (OEA). The results were compared to those obtained by the CFT and the competitive ELISA (cELISA). The tests were assessed with a panel of 17 serum samples from specific pathogen-free (SPF) lambs experimentally infected with various subtypes of
Chlamydophila pecorum, with sera from 45
C. abortus-infected pregnant sheep and from 54 sheep free of OEA. The
C. abortus ELISA was identified as being more specific and sensitive than the other tests. The 4 assays were evaluated further with 254 sera from flocks with documented OEA, from flocks with no history of abortion and from animals after abortion of unknown cause. The
C. abortus ELISA by the Institut Pourquier identified less OEA-positive sera than the other assays though it identified correctly 9 of 10 OEA-positive flocks. The basis of the discordant results is discussed.