Self-etching Increases Matrix Metalloproteinase Expression in the Dentin-Pulp Complex

• Published in: Journal of dental research Vol. 88; no. 1; pp. 77 - 82
• Main Authors: Lehmann, N., Debret, R., Roméas, A., Magloire, H., Degrange, M., Bleicher, F., Sommer, P., Seux, D.
• Format: Journal Article
• Language: English
• Published: London, England SAGE Publications 01-01-2009; SAGE PUBLICATIONS, INC; SAGE Publications (UK and US)
• Subjects: Adolescent; Blotting, Western; Coloring Agents; Composite Resins - chemistry; Composite Resins - pharmacology; Dental Pulp - drug effects; Dental Pulp - enzymology; Dental Pulp - ultrastructure; Dentin - drug effects; Dentin - enzymology; Dentin - ultrastructure; Dentin-Bonding Agents - chemistry; Dentin-Bonding Agents - pharmacology; Dentistry; Enzyme Precursors - drug effects; Gelatinases - drug effects; Humans; Immunohistochemistry; Isoenzymes - drug effects; Life Sciences; Light-Curing of Dental Adhesives; Materials Testing; Matrix Metalloproteinase 2 - drug effects; Matrix Metalloproteinase 9 - drug effects; Odontoblasts - drug effects; Odontoblasts - enzymology; Odontoblasts - ultrastructure; Surface Properties; Tissue Culture Techniques; acid-etched dentin; odontoblast; immunohistochemistry; matrix metalloproteinases; zymography
• ISSN: 0022-0345; 1544-0591
• DOI: 10.1177/0022034508327925

In adhesive restorations, one major problem is hybrid layer degradation. At present,
this deterioration is explained by the activation of the endogenous matrix
metalloproteinases (MMPs) present in dentin due to the acidic property of adhesive
systems. We hypothesized that self-etching adhesive should also stimulate the
expression of MMPs in odontoblasts. In cultured tooth slices, we evaluated the
changes in MMP-2 and proMMP-9 expression in the dentin-pulp complex after
self-etching adhesive treatment on dentin cavities in immunochemistry and by
zymography. The treatment resulted in increased MMP-2 expression in odontoblasts, as
shown by immunohistochemistry. Zymography showed increased proMMP-9 and MMP-2 in
dentin under self-etching treatment when pulp was present. These results showed that
self-etching adhesive stimulates the secretion of MMPs from the dentin-pulp complex
and, more precisely, by odontoblasts, suggesting that odontoblasts participate in
hybrid layer degradation.