Assessment of Antifungal Activity of Bakuchiol on Oral-Associated Candida spp.

• Published in: Evidence-based complementary and alternative medicine Vol. 2015; no. 2015; pp. 1 - 7
• Main Authors: Nordin, Mohd-Al-Faisal, Wan Harun, Wan Himratul Aznita, Abdul Razak, Fathilah
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01-01-2015; John Wiley & Sons, Inc; Hindawi Limited
• Subjects: Analysis; Antifungal agents; Biofilms; Candida; Colleges & universities; Metabolism; Metabolites; Proteins; Psoralea; Psoralea glandulosa; Seeds
• ISSN: 1741-427X; 1741-4288
• DOI: 10.1155/2015/918624

Bakuchiol is an active component of Psoralea glandulosa and Psoralea corylifolia, used in traditional Chinese medicine. The study aimed at investigating the antifungal activity of bakuchiol on planktonic and biofilm forms of orally associated Candida species. The antifungal susceptibility testing was determined by the broth micro dilution technique. Growth kinetics and cell surface hydrophobicity (CSH) of Candida were measured to assess the inhibitory effect of bakuchiol on Candida planktonic cells. Biofilm biomass and cellular metabolic activity were quantitatively estimated by the crystal violet (CV) and the 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT) assays. All Candida strains have been shown to be susceptible to bakuchiol with the MIC ranges from 12.5 to 100 μg/mL. Significant decrease in specific growth rates and viable counts demonstrates the inhibitory effect of bakuchiol on Candida planktonic cells. A brief exposure to bakuchiol also reduced CSH of Candida ( P < 0.05 ), indicating altered surface properties of yeast cells towards hydrophobic interfaces. Biofilm biomass and cell metabolic activity were mostly decreased, except for C. glabrata ( P = 0.29 ). The antifungal properties of bakuchiol on Candida species in this in vitro study may give insights into the application in therapeutic strategy against Candida infections.