Evaluation of the Antioxidant Properties of Carvacrol as a Prospective Replacement for Crude Essential Oils and Synthetic Antioxidants in Food Storage

• Published in: Molecules (Basel, Switzerland) Vol. 28; no. 3; p. 1315
• Main Authors: Ebhohimen, Israel Ehizuelen, Okolie, Ngozi P, Okpeku, Moses, Unweator, Mfon, Adeleke, Victoria T, Edemhanria, Lawrence
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 30-01-2023; MDPI
• Subjects: Additives; Affinity; Amino acids; antioxidant; Antioxidants; Antioxidants - chemistry; Butylated hydroxytoluene; Carvacrol; Chelating Agents; Chelation; Chemical properties; Enzymes; Essential oils; Food; Food additives; Food conservation; Food preservation; Food storage; Food Storage - methods; Lipid peroxidation; Lipids; Lipoxygenase; Lipoxygenases; Liquid oxygen; Materia medica, Vegetable; Methods; Molecular docking; Molecular Docking Simulation; Molecular dynamics; molecular dynamics simulation; Molecular interactions; Nitric oxide; Oils & fats; Oils, Volatile - chemistry; Oils, Volatile - pharmacology; Phenolic compounds; Phenols; Phytochemicals; Plant extracts; Prospective Studies; Protection and preservation; Proteins; Scavenging; Simulation; Toxicity; Nigeria; lipoxygenase; butylated hydroxytoluene; antioxidant; molecular docking; carvacrol; molecular dynamics simulation
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules28031315

The phenolic structural analogues of synthetic antioxidants such as butylated hydroxytoluene (BHT) in essential oils have been reported to exhibit antioxidant properties. Additionally, their lipophilicity makes them suitable for use in lipid-rich foods. This study evaluated the antioxidant capacity of carvacrol, a monoterpenoid antioxidant compound in the
(Gaertn.) seed essential oil, compared to the seed essential oil and BHT. In vitro studies (ferric reducing antioxidant power (FRAP), metal chelating activity (MCA), and nitric oxide scavenging activity (NOSA)) were conducted to ascertain if the antioxidant capacity of carvacrol was comparable to that of the seed essential oil. The potential binding affinity and molecular interactions between carvacrol and lipoxygenase (LOX) and its homologous model were investigated in silico. The molecular docking was performed using Autodock Vina, and the best poses were subjected to molecular dynamics simulation. The IC
for MCA and NOSA were: carvacrol 50.29 µL/mL, seed essential oil (SEO) 71.06 µL/mL; and carvacrol 127.61 µL/mL, SEO 165.18 µL/mL, respectively. The LOX model was Ramachandran favoured (97.75%) and the overall quality factor in the ERRAT plot was 95.392. The results of the molecular docking and molecular dynamics simulations revealed that lipoxygenase has a higher affinity (-22.79 kcal/mol) for carvacrol compared to BHT. In the LOX-BHT and LOX-carvacrol complexes, the root-mean-square deviation (RMSD), root-mean-square fluctuation (RMSF), and the radius of gyration (RoG) were not significantly different, indicating similar molecular interactions. The results obtained from this study suggest that carvacrol exhibits an antioxidant capacity that may be explored as an alternative for crude essential oils and synthetic compounds during the storage of lipid-rich foods.